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국내에서 2003년에 분리된 Eschcerichia coli와 Klebsiella pneumoniae의 CTX-M형 Extended-Spectrum β-Lactamase 생성 현황

Other Titles
 Prevalence of CTX-M-type Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae Isolates in Korea, 2003 
Authors
 이정현  ;  배일권  ;  용동은  ;  이경원  ;  김의종  ;  박연준  ;  어영  ;  신종희  ;  홍성근  ;  안지영  ;  강정옥  ;  이위교  ;  이종욱  ;  우건조  ;  정석훈  ;  권수봉 
Citation
 Korean Journal of Clinical Microbiology (대한임상미생물학회지), Vol.7(2) : 111-118, 2004 
Journal Title
 Korean Journal of Clinical Microbiology (대한임상미생물학회지) 
ISSN
 1229-0025 
Issue Date
2004
Keywords
Extended-Spectrum β-Lactamase ; CTX-M-3 ; CTX-M-14 ; CTX-M-15
Abstract
Background : The aims of this study were to survey nationwide susceptibilities of Escherichia coli and Klebsiella pneumoniae isolates against cefotaxime and to determine the prevalences of CTX-M type extended-spectrum β-lactamases(ESBLs). Methods : During the period of February to July, 2003, E. coli and K. pneumoniae isolates were collected from 12 hospitals. Antimicrobial susceptibilities to cefotaxime were tested by the disk diffusion method. ESBL production was determined by the double disk synergy test. Cefotaxime-resistance of the ESBL-producers was transfered to E. coli DH5α and E. coli Top10-F by transformation. MICs of β-lactam antibiotics were determined by the agar dilution method. Searches for blaCTX-M genes was performed by PCR amplication; pIs of β-lactamases were determined by isoelectric focusing. Results : Among 230 isolates of E. coli and 232 isolates of K. pneumoniae, 27 (11.7%) and 79 (34.1%) were intermediate or resistant to cefotaxime, respectively. Twenty-four(10.4%) isolates of E. coli and 58 (25.0%) K. pneumoniae isolates showed positive results in the double disk synergy test. Three isolates of E. coli and 13 K. pneumoniae isolates harbored blaCTX-M-3 gene, 4 E. coli isolates harbored blaCTX-M-15 gene, and 1 E. coli and 5 K. pneumoniae isolates harbored blaCTX-M-14 gene.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/111837
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