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가와사끼병 환아의 혈청 프로테옴 지도 비교

Other Titles
 Comparison of Serum Proteome Maps of Children with Kawasaki Disease 
Authors
 백승연  ;  이영목  ;  김동수  ;  이광훈 
Citation
 Korean Journal of Pediatrics, Vol.47(1) : 81-89, 2004 
Journal Title
 Korean Journal of Pediatrics 
ISSN
 1738-1061 
Issue Date
2004
Keywords
Kawasaki disease ; Proteomics ; Two-dimensional electrophoresis ; Proteome map
Abstract
Purpose : Kawasaki disease is a systemic vasculitis observed in children under five years of age and the leading cause of pediatric acquired heart disease, but its pathophysiology is still not completely understood. With proteomics, the study of quality and quantity of proteins, having been developed dramatically, we tried to find a way to evaluate the etiology and pathogenesis of Kawasaki disease by analyzing the serum proteome maps. Methods : We studied 10 patients admitted to the Pediatric Department of Yonsei Severance Hospital from January 2000 to July 2001. Half were diagnosed as typical Kawasaki disease and the rest as upper respiratory infection. Sera collected from the patients were frozen, melted and rehydrated for isoelectric focusing method using polyacrylamide gel and two-dimensional electrophoresis. The gel were stained by the silver method and scanned with GS-800 Calibrated Imaging Densitometer. PDQuest was used to quantify protein and draw proteome maps. Results : 15 protein spots of molecular weight(kDa)/isoelectric point 76.1/3.80, 75.0/4.70, 74.4/4.70, 66.3/5.77, 91.8/5.77, 125.5/5.84, 92.4/5.85, 83.8/6.09, 77.0/6.26, 76.8/6.39, 103.4/6.49, 126.5/6.40, 122.9/6.74, 35.3/6.18, 28.5/6.68 were significantly decreased in children with Kawasaki disease. Conclusion : Further evaluations using methods such as electrospray ionization mass spectrometry (ESI-MS), matrix-assisted laser desorption-ionization mass spectrometry(MALDI-MS) or SWISS- PROT(Swiss Institute of Bioinformatics, Geneva, Switzerland) would have to be performed to define and to understand the functions of those protein spots decreased in patients with Kawasaki disease.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Soo(김동수)
Baek, Seoung Yon(백승연)
Lee, Kwang Hoon(이광훈)
Lee, Young Mock(이영목) ORCID logo https://orcid.org/0000-0002-5838-249X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/111755
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