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Constitutive production of human leptin by fed-batch culture of recombinant rpoS−Escherichia coli

DC Field Value Language
dc.contributor.author금기창-
dc.contributor.author유내춘-
dc.contributor.author유원민-
dc.date.accessioned2015-07-14T16:50:30Z-
dc.date.available2015-07-14T16:50:30Z-
dc.date.issued2004-
dc.identifier.issn1046-5928-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/111697-
dc.description.abstractHigh-level production of human leptin by fed-batch culture of recombinant Escherichia coli using constitutive promoter system was investigated. For the constitutive expression of the obese gene encoding human leptin, the strong constitutive HCE promoter cloned from the d-amino acid aminotransferase gene of Geobacillus toebii was used. To develop an optimal host–vector system, several different recombinant E. coli strains were compared for leptin production. In flask cultures, E. coli FMJ123, which is a rpoS mutant strain, showed the highest level of leptin production (41% of total proteins). By comparing the expression levels of leptin in several different rpoS− and rpoS+ strains, it could be concluded that rpoS mutation positively affected constitutive production of leptin. For the large-scale production of human leptin, fed-batch cultures of recombinant E. coli FMJ123 were carried out using three different feeding solutions—chemically defined, yeast extract-containing, and casamino acid-containing feeding solutions. Among these, the use of casamino acid-containing feeding solution allowed production of leptin up to 2.1 g/L, which was 2.1- and 1.8-fold higher than that obtained with chemically defined and yeast extract-contained feeding solutions, respectively. These results suggest that the HCE promoter can be used for the efficient production of leptin, and most likely other recombinant proteins, in a constitutive manner.-
dc.description.statementOfResponsibilityopen-
dc.format.extent150~156-
dc.relation.isPartOfPROTEIN EXPRESSION AND PURIFICATION-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAlanine Transaminase/genetics-
dc.subject.MESHBacterial Proteins/genetics*-
dc.subject.MESHD-Alanine Transaminase-
dc.subject.MESHEscherichia coli/genetics*-
dc.subject.MESHEscherichia coli/metabolism-
dc.subject.MESHFermentation-
dc.subject.MESHGene Deletion-
dc.subject.MESHGenetic Vectors/genetics-
dc.subject.MESHHumans-
dc.subject.MESHLeptin/biosynthesis*-
dc.subject.MESHLeptin/genetics-
dc.subject.MESHPromoter Regions, Genetic/genetics-
dc.subject.MESHSigma Factor/genetics*-
dc.titleConstitutive production of human leptin by fed-batch culture of recombinant rpoS−Escherichia coli-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Radiation Oncology (방사선종양학)-
dc.contributor.googleauthorKi Jun Jeong-
dc.contributor.googleauthorJong Hyun Choi-
dc.contributor.googleauthorMoon-Hee Sung-
dc.contributor.googleauthorSang Yup Lee-
dc.contributor.googleauthorNae Choon Yoo-
dc.contributor.googleauthorKi Chang Keum-
dc.contributor.googleauthorWon Min Yoo-
dc.identifier.doi10.1016/j.pep.2004.04.007-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.relation.journalcodeJ02564-
dc.identifier.eissn1096-0279-
dc.identifier.pmid15177297-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S1046592804001214-
dc.subject.keywordConstitutive promoter-
dc.subject.keywordProtein expression-
dc.subject.keywordHuman leptin-
dc.subject.keywordHigh cell density culture-
dc.contributor.alternativeNameKeum, Ki Chang-
dc.contributor.alternativeNameYoo, Nae Choon-
dc.contributor.alternativeNameYoo, Won Min-
dc.rights.accessRightsnot free-
dc.citation.volume36-
dc.citation.number1-
dc.citation.startPage150-
dc.citation.endPage156-
dc.identifier.bibliographicCitationPROTEIN EXPRESSION AND PURIFICATION, Vol.36(1) : 150-156, 2004-
dc.identifier.rimsid37420-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers

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