Cited 30 times in
Determination of binding constant of transcription factor myc–max/max–max and E-box DNA: the effect of inhibitors on the binding
DC Field | Value | Language |
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dc.contributor.author | 박세연 | - |
dc.date.accessioned | 2015-07-14T16:49:03Z | - |
dc.date.available | 2015-07-14T16:49:03Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0006-3002 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/111649 | - |
dc.description.abstract | The truncated myc and max proteins, only containing basic regions and helix-loop-helix/zipper (b/HLH/Zip) regions were over-expressed in E. coli and used for the determination of the binding constant and of the inhibitory mechanism on myc-max (or max-max)-DNA complex formation. The association kinetic constants (k(1) and k(-1)) of truncated max-max or myc-max dimer and DNA were determined as k(1)=(1.7+/-0.6)x10(5) M(-1) s(-1), k(-1)=(3.4+/-1.2)x10(-2) s(-1) for max-max and DNA or k(1)=(2.1+/-0.7)x10(5) M(-1) s(-1), k(-1)=(3.2+/-1.4)x10(-2) s(-1) for myc-max and DNA. The equilibrium binding constant (K(1)) was determined using these kinetic parameters [K(XXD)=(7.8+/-2.6)x10(6) M(-1) for max-max and DNA or K(XYD)=(6.9+/-2.2)x10(6) M(-1) for myc-max and DNA]. The binding constants of myc-max or max-max dimer formation were K(XX)=(2.6+/-0.9)x10(5) M(-1) or K(XY)=(1.3+/-0.4)x10(4) M(-1), respectively. When truncated proteins were used, the max-max dimer formation was easier than the myc-max dimer formation, contrary to the physiologically determined case. This leads us to deduce that domains other than b/HLH/Zip are very important for the transcriptional regulatory activity in physiological conditions. The truncated myc and max proteins, which were expressed in E. coli and contained only b/HLH/Zip regions were also used for the screening of inhibitors of myc-max-DNA complex formation. A synthesized curcuminoid, 1,7-bis(4-methyl-3-nitrophenyl)-1,6-heptadiene-3,5-dione (curcuminoid 004), showed the most potent inhibition out of the synthesized curcuminoids, in competition with DNA. The dissociation constant of max-max dimer and the inhibitor was 9 microM, when investigated using in vitro expressed b/HLH/Zip dimer proteins. The curcuminoid 004 showed an inhibitory effect on the binding of myc-max protein to the E-box element in SNU16 cells, and suppressed the expression of myc target genes including ornithine decarboxylase (ODC), cdc25a and c-myc in myc over-expressed human stomach cancer cell line SNU16. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 217~228 | - |
dc.relation.isPartOf | BIOCHIMICA ET BIOPHYSICA ACTA | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Basic Helix-Loop-Helix Leucine Zipper Transcription Factors | - |
dc.subject.MESH | Basic-Leucine Zipper Transcription Factors | - |
dc.subject.MESH | Cell Line, Tumor/drug effects | - |
dc.subject.MESH | Curcumin/analogs & derivatives* | - |
dc.subject.MESH | Curcumin/pharmacology | - |
dc.subject.MESH | DNA-Binding Proteins/biosynthesis | - |
dc.subject.MESH | DNA-Binding Proteins/genetics | - |
dc.subject.MESH | DNA-Binding Proteins/metabolism* | - |
dc.subject.MESH | Dimerization | - |
dc.subject.MESH | Down-Regulation | - |
dc.subject.MESH | E-Box Elements*/drug effects | - |
dc.subject.MESH | Escherichia coli/genetics | - |
dc.subject.MESH | Escherichia coli/metabolism | - |
dc.subject.MESH | Gene Expression Regulation/drug effects | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Kinetics | - |
dc.subject.MESH | Leucine Zippers/genetics | - |
dc.subject.MESH | Masoprocol/pharmacology | - |
dc.subject.MESH | Models, Molecular | - |
dc.subject.MESH | Molecular Structure | - |
dc.subject.MESH | Oleanolic Acid/analogs & derivatives* | - |
dc.subject.MESH | Oleanolic Acid/pharmacology | - |
dc.subject.MESH | Protein Binding/drug effects | - |
dc.subject.MESH | Protein Structure, Tertiary | - |
dc.subject.MESH | Proto-Oncogene Proteins c-myc/biosynthesis | - |
dc.subject.MESH | Proto-Oncogene Proteins c-myc/genetics | - |
dc.subject.MESH | Proto-Oncogene Proteins c-myc/metabolism* | - |
dc.subject.MESH | Transcription Factors/chemistry | - |
dc.subject.MESH | Transcription Factors/metabolism* | - |
dc.subject.MESH | Transcription, Genetic/drug effects* | - |
dc.title | Determination of binding constant of transcription factor myc–max/max–max and E-box DNA: the effect of inhibitors on the binding | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Anesthesia and Pain Research Institute (마취통증의학연구소) | - |
dc.contributor.googleauthor | Seyeon Park | - |
dc.contributor.googleauthor | Sunah Chung | - |
dc.contributor.googleauthor | Chul-Hak Yang | - |
dc.contributor.googleauthor | Eun-Ryeong Hahm | - |
dc.contributor.googleauthor | Chihoon Park | - |
dc.contributor.googleauthor | Kyung-Chae Jung | - |
dc.contributor.googleauthor | Kyung-Mee Kim | - |
dc.identifier.doi | 10.1016/j.bbagen.2003.12.007 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.relation.journalcode | J00287 | - |
dc.identifier.eissn | 1878-2434 | - |
dc.identifier.pmid | 14980448 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S030441650300271X | - |
dc.subject.keyword | myc–max | - |
dc.subject.keyword | Binding constant | - |
dc.subject.keyword | Inhibitor | - |
dc.subject.keyword | SNU16 cell | - |
dc.subject.keyword | Target gene | - |
dc.contributor.alternativeName | Park, Se Yeon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 1670 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 217 | - |
dc.citation.endPage | 228 | - |
dc.identifier.bibliographicCitation | BIOCHIMICA ET BIOPHYSICA ACTA , Vol.1670(3) : 217-228, 2004 | - |
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