Cited 44 times in
Smooth muscle-like tissues engineered with bone marrow stromal cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김일권 | - |
dc.date.accessioned | 2015-07-14T16:48:08Z | - |
dc.date.available | 2015-07-14T16:48:08Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/111618 | - |
dc.description.abstract | Bone marrow-derived cells have demonstrated the ability to differentiate into multiple mesenchymal cell lineages. Here we tested whether smooth muscle (SM)-like tissues can be created in vivo with bone marrow stromal cells (BMSCs). Cultured canine BMSCs, which expressed SM cell-specific markers including SM α-actin and SM myosin heavy chain, were seeded on three-dimensional, biodegradable polymer scaffolds and implanted into peritoneal cavity of athymic mice. The cell–scaffold constructs retrieved 4 weeks after implantation formed three-dimensional tissues. Immunohistochemical analyses showed that the tissue reconstructs expressed SM α-actin and SM myosin heavy chain. Masson’s trichrome staining showed the presence of significant amounts of collagen in the tissue reconstructs. Cells labeled with a fluorescent tracer prior to implantation were still present in the tissue reconstructs 4 weeks after implantation. Non-seeded scaffolds (control groups) retrieved 4 weeks after implantation did not exhibit extensive tissue formation. This study demonstrates the potential of BMSCs as an alternative cell source for tissue engineering of SM. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 2979~2986 | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Absorbable Implants* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | BoneMarrowCells/cytology* | - |
dc.subject.MESH | BoneMarrowCells/physiology* | - |
dc.subject.MESH | BoneMarrowTransplantation/methods* | - |
dc.subject.MESH | Cell Culture Techniques/methods | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Cell Division | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Dogs | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Nude | - |
dc.subject.MESH | Myocytes,SmoothMuscle/cytology* | - |
dc.subject.MESH | Myocytes,SmoothMuscle/physiology* | - |
dc.subject.MESH | StromalCells/cytology | - |
dc.subject.MESH | StromalCells/physiology | - |
dc.subject.MESH | StromalCells/transplantation | - |
dc.subject.MESH | Tissue Engineering/methods* | - |
dc.title | Smooth muscle-like tissues engineered with bone marrow stromal cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | Seung-Woo Cho | - |
dc.contributor.googleauthor | Il-Kwon Kim | - |
dc.contributor.googleauthor | Byung-Soo Kim | - |
dc.contributor.googleauthor | Cha Yong Choi | - |
dc.contributor.googleauthor | Eun Yeol Lee | - |
dc.contributor.googleauthor | Young Ha Kim | - |
dc.contributor.googleauthor | Soo Hyun Kim | - |
dc.contributor.googleauthor | Sun-Woong Kang | - |
dc.contributor.googleauthor | Dong-Ik Kim | - |
dc.contributor.googleauthor | Sang Hyun Lim | - |
dc.identifier.doi | 10.1016/j.biomaterials.2003.09.068 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.relation.journalcode | J00312 | - |
dc.identifier.eissn | 1878-5905 | - |
dc.identifier.pmid | 14967530 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S014296120300810X | - |
dc.subject.keyword | Bone marrow stromal cells | - |
dc.subject.keyword | Smooth muscle | - |
dc.subject.keyword | Tissue engineering | - |
dc.subject.keyword | Biodegradable polymer scaffold | - |
dc.contributor.alternativeName | Kim, Il Kwon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 25 | - |
dc.citation.number | 15 | - |
dc.citation.startPage | 2979 | - |
dc.citation.endPage | 2986 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, Vol.25(15) : 2979-2986, 2004 | - |
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