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Importance of integrin β1-mediated cell adhesion on biodegradable polymers under serum depletion in mesenchymal stem cells and chondrocytes

 Jin Woo Lee  ;  Yun Hee Kim  ;  Soo Bong Hahn  ;  Jung Woog Shin  ;  Kyoung Soo Jee  ;  Ki Dong Park 
 BIOMATERIALS, Vol.25(10) : 1901-1909, 2004 
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Cell adhesion ; Integrin β1 ; Mesenchymal stem cell ; Chondrocyte ; Surface roughness ; Contact angle
To evaluate the predominant mechanism of chondrogenic cell [mesenchymal stem cells (MSCs) and chondrocytes] adhesion under serum free conditions, we measured the surface roughness and wettability of poly(lactic acid:polyglycolic acid=75:25) (PLGA), poly(lactic acid) (PLA), and poly(-epsilon-caprolactone) (PCL)-coated glass plates. Also to evaluate the biological reactions involved in cell–polymer interactions, integrin β1, one of the cell adhesion molecules, was blocked with monoclonal antibody. In cell attachment test, MSCs and chondrocytes adhesion to synthetic polymers in 1 h were very low and ranged from 2.8% to 8.0%. In present study, the correlation between attachment rate and surface roughness, contact angle, or integrin β1 blocking on PLGA, PLA and PCL-coated plates could not be proved. However, we found that l-arginine-coated PLA highly increased the attachment rates of MSCs (30.2%) and of chondrocytes (26%), whereas integrin β1 blocking significantly decreased these attachment rates to 5.6% and 7.4%, respectively, suggesting that increased cell adhesion to l-arginine-coated plates is mediated by integrin β1. In this study, we showed that polymer characteristics such as roughness and wettability did not play an important role in cell adhesion under serum free conditions, because there was no significant difference according to polymer characteristics, whereas biological interactions mediated by integrin β1 were critical during the early period of cell adhesion. The results suggest that l-arginine could be useful for facilitating early cell adhesion to synthetic polymers in cartilage tissue engineering.
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1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
Hahn, Soo Bong(한수봉)
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