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Effect of Chlorophyllin on Normothermic Storage of Human Periodontal Ligament Cells

Authors
 Won-Gyun Chung  ;  Eun Ju Lee  ;  Jin Kim  ;  Seung-Ae Lee  ;  Seung-Jong Lee 
Citation
 JOURNAL OF ENDODONTICS, Vol.30(6) : 399-402, 2004 
Journal Title
 JOURNAL OF ENDODONTICS 
ISSN
 0099-2399 
Issue Date
2004
MeSH
Antioxidants/pharmacology* ; Cell Survival/drug effects ; Cells, Cultured ; Chlorophyllides/pharmacology* ; Coloring Agents ; Culture Media ; Dose-Response Relationship, Drug ; Flow Cytometry ; G1 Phase ; Humans ; Isotonic Solutions/pharmacology ; Periodontal Ligament/cytology ; Periodontal Ligament/drug effects* ; Resting Phase, Cell Cycle ; Temperature ; Tetrazolium Salts ; Thiazoles ; Tissue Preservation*
Abstract
The purpose of the present study was to evaluate whether chlorophyllin could serve as an effective constituent of a storage medium to enhance the human periodontal ligament (PDL) cell viability. Freshly isolated PDL cells from premolars extracted from healthy people were stored at 37 degrees C for 6 h in various solutions: F-medium and Hank's balanced salt solution (HBSS), supplemented with chlorophyllin. From MTT viability assays, the highest cell viability was found in the PDL cells stored in HBSS supplemented with 500 nM chlorophyllin, and the chlorophyllin-treated cells showed a dose-dependent response to concentration. Additionally, the results from flow cytometry showed that 77 to 80% of the PDL cells were in the G0/G1 phases of the cell cycle, which suggested that most were in a stable stage. These result showed that HBSS, supplemented with chlorophyllin, may be a useful solution for preserving the viability of PDL cells.
Full Text
http://www.sciencedirect.com/science/article/pii/S0099239905602014
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jin(김진)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/111616
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