0 160

Cited 9 times in

Molecular cloning and biochemical characterization of Candida albicans acyl-CoA:sterol acyltransferase, a potential target of antifungal agents

DC FieldValueLanguage
dc.contributor.author박종철-
dc.date.accessioned2015-07-14T16:46:07Z-
dc.date.available2015-07-14T16:46:07Z-
dc.date.issued2004-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/111550-
dc.description.abstractTo determine whether Candida albicans acyl CoA:sterol acyltransferase (ASAT) can be a potential target enzyme for the protoberberine derivative (HWY-289), we have isolated a gene encoding Ca-ASAT and examined inhibitory effects of HWY-289 on the overexpressed Ca-ASAT. HWY-289 specifically inhibits Ca-ASAT in a non-competitive manner in vitro (IC50 [9.2 μM], Ki [5.15 μM]). The cloned CaARE2 gene (1830 nucleotides [nt]) encodes active Ca-ASAT protein that exhibits a calculated molecular mass of 71.3 kDa. The amino acid sequence of CaAre2p is 33.4% and 35.1% identical to those of Saccharomyces cerevisiae ScAre1p and ScAre2p homologues, respectively. Recombinant and endogenous Ca-ASAT displayed identical patterns of inhibition upon exposure to HWY-289 and a preference for cholesterol and oleoyl-CoA as substrates. Northern blot analysis showed that CaARE2 was activated by HWY-289, but not by CI-976 (a human acyl-coenzyme A:cholesterol acyltransferase inhibitor), in a dose-dependent manner (up to 5 mg/L), suggesting different selectivities of action between HWY-289 and CI-976 on Ca-ASAT activity.-
dc.description.statementOfResponsibilityopen-
dc.format.extent911~919-
dc.relation.isPartOfBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleMolecular cloning and biochemical characterization of Candida albicans acyl-CoA:sterol acyltransferase, a potential target of antifungal agents-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Medical Engineering (의학공학)-
dc.contributor.googleauthorYoung-Ki Paik-
dc.contributor.googleauthorKi-Young Kim-
dc.contributor.googleauthorDong-Min Han-
dc.contributor.googleauthorHongyuan Yang-
dc.contributor.googleauthorJung-Ho Kim-
dc.contributor.googleauthorJong-Chul Park-
dc.contributor.googleauthorYu-Kyong Shin-
dc.identifier.doi10.1016/j.bbrc.2004.05.076-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.relation.journalcodeJ00281-
dc.identifier.eissn1090-2104-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X04010435-
dc.subject.keywordAcyl-coenzyme A:sterol acyltransferase-
dc.subject.keywordErgosterol-
dc.subject.keywordSterol ester-
dc.subject.keywordCandida albicans-
dc.subject.keywordAntifungal agent-
dc.subject.keywordHWY-289-
dc.contributor.alternativeNamePark, Jong Chul-
dc.rights.accessRightsnot free-
dc.citation.volume319-
dc.citation.number3-
dc.citation.startPage911-
dc.citation.endPage919-
dc.identifier.bibliographicCitationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.319(3) : 911-919, 2004-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.