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Helicobacter pylori in a Korean isolate activates mitogen-activated protein kinases, AP-1, and NF-κB and induces chemokine expression in gastric epithelial AGS cells

DC FieldValueLanguage
dc.contributor.author김경환-
dc.contributor.author서지혜-
dc.contributor.author임주원-
dc.date.accessioned2015-07-14T16:45:21Z-
dc.date.available2015-07-14T16:45:21Z-
dc.date.issued2004-
dc.identifier.issn0023-6837-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/111525-
dc.description.abstractOxidant-sensitive transcription factors, nuclear factor-kappaB (NF-kappaB), and activator protein-1 (AP-1) have been considered as the regulators of inducible genes such as chemokines. Since oxygen radicals are considered as an important regulator in the pathogenesis of Helicobacter pylori (H. pylori)-induced gastric ulceration and carcinogenesis, chemokines such as interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) may be regulated by NF-kappaB and/or AP-1. Ras, the upstream activator for mitogen-activated protein kinase (MAPK) and MAPK cascade regulate AP-1 activation. The present study aims to investigate whether H. pylori in a Korean isolate (HP99) induces the expression of chemokines (IL-8, MCP-1), which is regulated by Ras, MAPK, AP-1, and NF-kappaB in gastric epithelial AGS cells, and whether these transcriptional regulations of chemokines are inhibited by transfection with mutant genes for Ras (ras N-17), c-Jun (TAM-67), and IkappaBalpha (MAD-3) or treatment with MAPK inhibitors (U0126 for extracellular signal-regulated kinase or SB203580 for p38 kinase). In addition, virulence factors of HP99 were characterized by PCR analysis for the isolated DNA. As a result, HP99 is identified as cagA+, vacA s1b, m2, iceA1 H. pylori strain. HP99 induced a time-dependent expression of mRNA and protein for IL-8 and MCP-1 via mediation of MAPK, AP-1, and NF-kappaB. Transfection with mutant genes for Ras, c-Jun, and IkappaBalpha and treatment with MAPK inhibitors suppressed H. pylori-induced activation of transcription factors (NF-kappaB, AP-1) and expression of chemokines (IL-8, MCP-1) in AGS cells. In conclusion, Ras and MAPK cascade may act as the upstream signaling for the activation of AP-1 and NF-kappaB, which induce chemokine expression in H. pylori-infected AGS cells. Specific targeting of the activation of NF-kappaB and AP-1 may be effective for the prevention or treatment of gastric inflammation associated with H. pylori infection.-
dc.description.statementOfResponsibilityopen-
dc.format.extent49~62-
dc.relation.isPartOfLABORATORY INVESTIGATION-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleHelicobacter pylori in a Korean isolate activates mitogen-activated protein kinases, AP-1, and NF-κB and induces chemokine expression in gastric epithelial AGS cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학)-
dc.contributor.googleauthorJi Hye Seo-
dc.contributor.googleauthorJoo Weon Lim-
dc.contributor.googleauthorKyung Hwan Kim-
dc.contributor.googleauthorHyeyoung Kim-
dc.identifier.doi10.1038/labinvest.3700010-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.relation.journalcodeJ02150-
dc.identifier.eissn1530-0307-
dc.identifier.urlhttp://www.nature.com/labinvest/journal/v84/n1/full/3700010a.html-
dc.subject.keywordHelicobacter pylori-
dc.subject.keywordchemokine-
dc.subject.keywordnuclear factor-kB-
dc.subject.keywordmitogen-activated protein kinase-
dc.subject.keywordactivator protein-1-
dc.subject.keywordgastric epithelial AGS cells-
dc.contributor.alternativeNameKim, Kyung Hwan-
dc.contributor.alternativeNameSeo, Ji Hye-
dc.contributor.alternativeNameLim, Joo Weon-
dc.rights.accessRightsnot free-
dc.citation.volume84-
dc.citation.number1-
dc.citation.startPage49-
dc.citation.endPage62-
dc.identifier.bibliographicCitationLABORATORY INVESTIGATION, Vol.84(1) : 49-62, 2004-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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