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Effects of Human Embryonic Stem Cell Transplantation withSingle or Colony Form on the Functional Recovery inSpinal Cord Injured Rats

 Kye-Seong Kim  ;  Je Kyung Seong  ;  Bae Hwan Lee  ;  Se Jung Jung  ;  Un Jeng Kim  ;  Eun Joo Kim 
 Journal of Applied Micromagnetic Energy (응용미약에너지학회지), Vol.2(1) : 18-24, 2004 
Journal Title
Journal of Applied Micromagnetic Energy(응용미약에너지학회지)
Issue Date
Spinal cord injury ; embryonic stem cell ; transplantation ; functional recovery
Embryonic stem (ES) cells derived from the inner cell mass of blastocyst-stage embryos are totipotent.Recent studies indicated the potential use of ES cells on transplantation in the central nervous system with different typeof injuries. The present study was conducted to investigate functional recovery following transplantation of human EScells with single or colony form after SCI. Male Sprague-Dawley rats were anesthetized with sodium pentobarbital andsubjected to SCI model. Rats were laminectomized and SCI was induced using NYU spinal impactor at T9 spinal seg-ment. Human ES (HES) cells with single or colony form were transplanted 1 week after SCI. A behavioral test usingBBB locomotor rating scaling was performed. HES cells transplanted into rats promoted the hindlimb performance, anHES cell transplant significantly improves locomotion recovery. Especially, 2×104 HES cells with single form greatlyimproved hindlimb performance but colony-form did not. Histologically, 2×104 HES cells tended to fill the cavity butsingle form showed more reduced cavity size compared to colony. These results suggest that HES cells with single formare more effective in function recovery after transplantation in spinal cord injured rats compared to colony form.
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1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Un Jeng(김은정) ORCID logo https://orcid.org/0000-0003-0968-0252
Lee, Bae Hwan(이배환) ORCID logo https://orcid.org/0000-0003-4719-9021
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