Cited 26 times in
Daxx deletion mutant (amino acids 501-625)-induced apoptosis occurs through the JNK/p38-Bax-dependent mitochondrial pathway
DC Field | Value | Language |
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dc.contributor.author | 송재진 | - |
dc.date.accessioned | 2015-07-14T16:38:58Z | - |
dc.date.available | 2015-07-14T16:38:58Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0730-2312 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/111311 | - |
dc.description.abstract | Death-associated protein (Daxx) deletion mutant (aa 501–625) has been known to be an inducer of apoptosis. In this study, we observed that the Bax-dependent mitochondrial death signaling pathway plays an important role in Daxx501–625-induced apoptosis. Daxx fragment-induced activation of caspase-9 and -3 was mediated through the apoptosis signal-regulating kinase 1 (ASK1)–MEK–c-Jun-N-terminal kinase (JNK)/p38–Bax pathway. By overexpressing JNK-binding domain (JBD) of JIP1, a JNK-inhibitory protein, and treatment with SB203580, a specific p38 inhibitor, DU-145 cells were made resistant to Daxx501–625-induced apoptosis. Capase-3 deficiency, Bax deficiency, or overexpression of a dominant-negative caspase-9 mutant prevented apoptosis, even though the Daxx501–625 fragment still activated the ASK1–MEK–MAPK pathway. Interestingly, Daxx501–625-induced Bcl-2 interacting domain (Bid) cleavage was suppressed in the dominant-negative caspase-9 mutant cells, whereas Bim was still phosphorylated in these cells. These results suggest that cleavage of Bid occurs downstream of caspase-9 activation. In contrast, phosphorylation of Bim is upstream of caspase-9 activation. Taken together, our results suggest that Daxx501–625-induced apoptosis is mediated through the ASK1–MEK–JNK/p38–Bim–Bax-dependent caspase pathway. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1257~1270 | - |
dc.relation.isPartOf | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adaptor Proteins, Signal Transducing | - |
dc.subject.MESH | Adenoviridae/genetics | - |
dc.subject.MESH | Apoptosis/physiology* | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Carrier Proteins/genetics | - |
dc.subject.MESH | Carrier Proteins/physiology* | - |
dc.subject.MESH | Caspase 9 | - |
dc.subject.MESH | Caspases/metabolism | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cytochromes c/metabolism | - |
dc.subject.MESH | DNA Primers | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Genetic Vectors | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Intracellular Signaling Peptides and Proteins/genetics | - |
dc.subject.MESH | Intracellular Signaling Peptides and Proteins/physiology* | - |
dc.subject.MESH | JNKMitogen-Activated Protein Kinases/metabolism* | - |
dc.subject.MESH | MAP Kinase Kinase 4 | - |
dc.subject.MESH | Mitochondria/enzymology | - |
dc.subject.MESH | Mitochondria/metabolism* | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase Kinases/metabolism* | - |
dc.subject.MESH | Mutation | - |
dc.subject.MESH | Nuclear Proteins/genetics | - |
dc.subject.MESH | Nuclear Proteins/physiology* | - |
dc.subject.MESH | Proto-Oncogene Proteins c-bcl-2/metabolism* | - |
dc.subject.MESH | SequenceDeletion | - |
dc.subject.MESH | bcl-2-Associated X Protein | - |
dc.subject.MESH | p38 Mitogen-Activated Protein Kinases/metabolism* | - |
dc.title | Daxx deletion mutant (amino acids 501-625)-induced apoptosis occurs through the JNK/p38-Bax-dependent mitochondrial pathway | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Institute for Cancer Research (암연구소) | - |
dc.contributor.googleauthor | Jae J. Song | - |
dc.contributor.googleauthor | Yong J. Lee | - |
dc.identifier.doi | 10.1002/jcb.20155 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.relation.journalcode | J01303 | - |
dc.identifier.eissn | 1097-4644 | - |
dc.identifier.pmid | 15258908 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/jcb.20155/abstract | - |
dc.subject.keyword | Daxx | - |
dc.subject.keyword | ASK1 | - |
dc.subject.keyword | JNK | - |
dc.subject.keyword | Bax | - |
dc.subject.keyword | Bim | - |
dc.subject.keyword | cytochrome c | - |
dc.subject.keyword | caspase | - |
dc.contributor.alternativeName | Song, Jae Jin | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 92 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1257 | - |
dc.citation.endPage | 1270 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELLULAR BIOCHEMISTRY, Vol.92(6) : 1257-1270, 2004 | - |
dc.identifier.rimsid | 35973 | - |
dc.type.rims | ART | - |
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