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Zn2+ -induced cell death is mediated by the induction of intracellular ROS in ARPE-19 cells

DC FieldValueLanguage
dc.contributor.author고형준-
dc.contributor.author권오웅-
dc.contributor.author김성수-
dc.contributor.author김응권-
dc.contributor.author이성철-
dc.contributor.author이준행-
dc.date.accessioned2015-07-14T16:35:07Z-
dc.date.available2015-07-14T16:35:07Z-
dc.date.issued2004-
dc.identifier.issn0271-3683-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/111187-
dc.description.abstractPURPOSE: Recent studies have shown that Zn2+ induced cell death in retinal pigment epithelial cells. Here we sought to investigate the mode of Zn2+-induced cell death and the role of reactive oxygen species (ROS) in human retinal pigment epithelial cell line, ARPE-19 cells. METHODS: Cell viability was measured by MTT assay. Cell death of ARPE-19 cells was measured by annexin V-fluorescein isothiocyanate (FITC) binding assay, TUNEL assay. The formation of intracellular ROS was measured using 2',7'-dichlorofluorescein diacetate (DCFH-DA). The activation of mitogen-activated protein kinase (MAPK) was examined by Western blot analysis. RESULTS: This study demonstrated that Zn2+ treatment induced both necrosis and apoptosis in ARPE-19 cells. Exposure of ARPE-19 cells to Zn2+ led to the activation of ERK1/2, JNK1/2/3, and p38 MAPKs. The activation of these MAPKs was blocked by treatment with the antioxidant, N-acetylcystein (NAC). More importantly, inhibition of ROS production by NAC completely prevented Zn2+-induced cell death in RPE cells. CONCLUSIONS: This study suggests that Zn2+ induces both apoptosis and necrosis in ARPE-19 cells and that its cytotoxicity may depend on the induction of intracellular ROS.-
dc.description.statementOfResponsibilityopen-
dc.format.extent195~201-
dc.relation.isPartOfCurrent Eye Research-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleZn2+ -induced cell death is mediated by the induction of intracellular ROS in ARPE-19 cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Ophthalmology (안과학)-
dc.contributor.googleauthorJeongmin Song-
dc.contributor.googleauthorSung Chul Lee-
dc.contributor.googleauthorJoon H. Lee-
dc.contributor.googleauthorSeung-Hun Shin-
dc.contributor.googleauthorEung Kweon Kim-
dc.contributor.googleauthorJimmy Jaeyoung Kang-
dc.contributor.googleauthorOh Woong Kwon-
dc.contributor.googleauthorHyung J. Koh-
dc.contributor.googleauthorSung Soo Kim-
dc.identifier.doi10.1076/ceyr.28.3.195.26251-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00571-
dc.contributor.localIdA00152-
dc.contributor.localIdA00235-
dc.contributor.localIdA00831-
dc.contributor.localIdA02873-
dc.contributor.localIdA03180-
dc.relation.journalcodeJ00665-
dc.identifier.urlhttp://informahealthcare.com/doi/abs/10.1076/ceyr.28.3.195.26251-
dc.contributor.alternativeNameKoh, Hyoung Jun-
dc.contributor.alternativeNameKwon, Oh Woong-
dc.contributor.alternativeNameKim, Sung Soo-
dc.contributor.alternativeNameKim, Eung Kweon-
dc.contributor.alternativeNameLee, Sung Chul-
dc.contributor.alternativeNameLee, Joon Haeng-
dc.contributor.affiliatedAuthorKim, Sung Soo-
dc.contributor.affiliatedAuthorKoh, Hyoung Jun-
dc.contributor.affiliatedAuthorKwon, Oh Woong-
dc.contributor.affiliatedAuthorKim, Eung Kweon-
dc.contributor.affiliatedAuthorLee, Sung Chul-
dc.contributor.affiliatedAuthorLee, Joon Haeng-
dc.rights.accessRightsnot free-
dc.citation.volume28-
dc.citation.number3-
dc.citation.startPage195-
dc.citation.endPage201-
dc.identifier.bibliographicCitationCurrent Eye Research, Vol.28(3) : 195-201, 2004-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers

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