Cited 21 times in
Zn2+ -induced cell death is mediated by the induction of intracellular ROS in ARPE-19 cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 고형준 | - |
dc.contributor.author | 권오웅 | - |
dc.contributor.author | 김성수 | - |
dc.contributor.author | 김응권 | - |
dc.contributor.author | 이성철 | - |
dc.contributor.author | 이준행 | - |
dc.date.accessioned | 2015-07-14T16:35:07Z | - |
dc.date.available | 2015-07-14T16:35:07Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0271-3683 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/111187 | - |
dc.description.abstract | PURPOSE: Recent studies have shown that Zn2+ induced cell death in retinal pigment epithelial cells. Here we sought to investigate the mode of Zn2+-induced cell death and the role of reactive oxygen species (ROS) in human retinal pigment epithelial cell line, ARPE-19 cells. METHODS: Cell viability was measured by MTT assay. Cell death of ARPE-19 cells was measured by annexin V-fluorescein isothiocyanate (FITC) binding assay, TUNEL assay. The formation of intracellular ROS was measured using 2',7'-dichlorofluorescein diacetate (DCFH-DA). The activation of mitogen-activated protein kinase (MAPK) was examined by Western blot analysis. RESULTS: This study demonstrated that Zn2+ treatment induced both necrosis and apoptosis in ARPE-19 cells. Exposure of ARPE-19 cells to Zn2+ led to the activation of ERK1/2, JNK1/2/3, and p38 MAPKs. The activation of these MAPKs was blocked by treatment with the antioxidant, N-acetylcystein (NAC). More importantly, inhibition of ROS production by NAC completely prevented Zn2+-induced cell death in RPE cells. CONCLUSIONS: This study suggests that Zn2+ induces both apoptosis and necrosis in ARPE-19 cells and that its cytotoxicity may depend on the induction of intracellular ROS. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 195~201 | - |
dc.relation.isPartOf | CURRENT EYE RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Zn2+ -induced cell death is mediated by the induction of intracellular ROS in ARPE-19 cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학) | - |
dc.contributor.googleauthor | Jeongmin Song | - |
dc.contributor.googleauthor | Sung Chul Lee | - |
dc.contributor.googleauthor | Joon H. Lee | - |
dc.contributor.googleauthor | Seung-Hun Shin | - |
dc.contributor.googleauthor | Eung Kweon Kim | - |
dc.contributor.googleauthor | Jimmy Jaeyoung Kang | - |
dc.contributor.googleauthor | Oh Woong Kwon | - |
dc.contributor.googleauthor | Hyung J. Koh | - |
dc.contributor.googleauthor | Sung Soo Kim | - |
dc.identifier.doi | 10.1076/ceyr.28.3.195.26251 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00571 | - |
dc.contributor.localId | A00152 | - |
dc.contributor.localId | A00235 | - |
dc.contributor.localId | A00831 | - |
dc.contributor.localId | A02873 | - |
dc.contributor.localId | A03180 | - |
dc.relation.journalcode | J00665 | - |
dc.identifier.eissn | 1460-2202 | - |
dc.identifier.url | http://informahealthcare.com/doi/abs/10.1076/ceyr.28.3.195.26251 | - |
dc.subject.keyword | apoptosis | - |
dc.subject.keyword | mitogen-activated protein kinases | - |
dc.subject.keyword | necrosis | - |
dc.subject.keyword | reactive oxygen species | - |
dc.subject.keyword | Zn2+ | - |
dc.contributor.alternativeName | Koh, Hyoung Jun | - |
dc.contributor.alternativeName | Kwon, Oh Woong | - |
dc.contributor.alternativeName | Kim, Sung Soo | - |
dc.contributor.alternativeName | Kim, Eung Kweon | - |
dc.contributor.alternativeName | Lee, Sung Chul | - |
dc.contributor.alternativeName | Lee, Joon Haeng | - |
dc.contributor.affiliatedAuthor | Kim, Sung Soo | - |
dc.contributor.affiliatedAuthor | Koh, Hyoung Jun | - |
dc.contributor.affiliatedAuthor | Kwon, Oh Woong | - |
dc.contributor.affiliatedAuthor | Kim, Eung Kweon | - |
dc.contributor.affiliatedAuthor | Lee, Sung Chul | - |
dc.contributor.affiliatedAuthor | Lee, Joon Haeng | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 28 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 195 | - |
dc.citation.endPage | 201 | - |
dc.identifier.bibliographicCitation | CURRENT EYE RESEARCH, Vol.28(3) : 195-201, 2004 | - |
dc.identifier.rimsid | 36194 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.