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Melatonin prevents nitric oxide-induced apoptosis by increasing the interaction between 14-3-3beta and p-Bad in SK-N-MC cells

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dc.contributor.author최승일-
dc.date.accessioned2015-06-11T14:11:41Z-
dc.date.available2015-06-11T14:11:41Z-
dc.date.issued2008-
dc.identifier.issn0742-3098-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/111144-
dc.description.abstractThe anti-apoptotic effect of melatonin has been described in vivo and in vitro. A previous report has revealed that melatonin suppresses nitric oxide (NO)-induced apoptosis via the induction of Bcl-2 expression in PGT-beta pineal cells. To investigate the protective mechanism of melatonin on NO donor S-nitroso-N-acetyl-penicillamine (SNAP)-induced apoptosis, we examined the anti-apoptotic upstream signaling pathway of Bcl-2 in the human neuroblastoma cell line SK-N-MC. The flow cytometry results revealed that apoptosis occurred in NO-treated cells, while cell death was inhibited by pretreatment with melatonin (100 microm). In addition, decreased Bax expression, increased Bcl-2 expression and a decreased release of cytochrome c into the cytosol were observed in the melatonin-pretreated SK-N-MC cells. We also found that melatonin treatment induced the activation of Akt/PKB and the phosphorylation of GSK3alpha/beta and Bad. Furthermore, melatonin treatment not only increased the protein-protein interactions between 14-3-3beta and p-Bad, but also decreased the release of cytochrome c from mitochondria into the cytosol. In summary, the protective effect of melatonin against NO-induced apoptosis was mediated by the inhibition of Bad translocation from the cytosol to the mitochondria by the induction of protein-protein interactions between 14-3-3beta and p-Bad-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJOURNAL OF PINEAL RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESH14-3-3 Proteins/metabolism*-
dc.subject.MESHApoptosis/drug effects*-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHHumans-
dc.subject.MESHMelatonin/pharmacology*-
dc.subject.MESHNitric Oxide/adverse effects-
dc.subject.MESHProto-Oncogene Proteins c-akt/metabolism*-
dc.subject.MESHSignal Transduction/drug effects-
dc.subject.MESHbcl-Associated Death Protein/metabolism*-
dc.titleMelatonin prevents nitric oxide-induced apoptosis by increasing the interaction between 14-3-3beta and p-Bad in SK-N-MC cells-
dc.typeArticle-
dc.contributor.collegeResearcher Institutes (부설 연구소)-
dc.contributor.departmentCorneal Dystrophy Research Institute (각막이상증연구소)-
dc.contributor.googleauthorSeung-Il Choi-
dc.contributor.googleauthorSeong-Soo Joo-
dc.contributor.googleauthorYeong-Min Yoo-
dc.identifier.doi10.1111/j.1600-079X.2007.00494.x-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA04099-
dc.relation.journalcodeJ01712-
dc.identifier.eissn1600-079X-
dc.identifier.pmid18078454-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/j.1600-079X.2007.00494.x/abstract-
dc.subject.keywordAkt/PKB-
dc.subject.keywordBad-
dc.subject.keywordGSK3 a/b-
dc.subject.keywordmelatonin-
dc.subject.keywordSK-N-MC-
dc.subject.keywordSNAP-
dc.subject.keyword14-3-3b-
dc.contributor.alternativeNameChoi, Seung Il-
dc.contributor.affiliatedAuthorChoi, Seung Il-
dc.rights.accessRightsnot free-
dc.citation.volume44-
dc.citation.number1-
dc.citation.startPage95-
dc.citation.endPage100-
dc.identifier.bibliographicCitationJOURNAL OF PINEAL RESEARCH, Vol.44(1) : 95-100, 2008-
dc.identifier.rimsid42590-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers

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