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Enhancement of in vivo endothelialization of tissue-engineered vascular grafts by granulocyte colony-stimulating factor

Authors
 Seung-Woo Cho  ;  Joung Eun Lim  ;  Hun Su Chu  ;  Hye-Jin Hyun  ;  Cha Yong Choi  ;  Ki-Chul Hwang  ;  Kyung Jong Yoo  ;  Dong-Ik Kim  ;  Byung-Soo Kim 
Citation
 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, Vol.76A(2) : 252-263, 2006 
Journal Title
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
ISSN
 1549-3296 
Issue Date
2006
MeSH
Animals ; Aorta ; Blood Vessel Prosthesis* ; Bone Marrow Cells/cytology ; Cell Differentiation ; Dogs ; Endothelial Cells/cytology ; Granulocyte Colony-Stimulating Factor/administration & dosage ; Granulocyte Colony-Stimulating Factor/pharmacology* ; Myocytes, Smooth Muscle/cytology ; Prosthesis Implantation ; Regeneration/drug effects* ; Tissue Engineering/methods*
Keywords
tissue‐engineered vascular graft ; bone marrow–derived cell ; decellularized tissue matrix ; granulocyte colony‐stimulating factor ; endothelialization
Abstract
Successful reconstruction of large-diameter blood vessel in humans has been demonstrated using the tissue engineering technique, but improvement in patency of small-diameter bioartificial vascular graft remains a great challenge. This study reports that granulocyte colony-stimulating factor (G-CSF) can enhance in vivo endothelialization of tissue-engineered vascular grafts, which could be used to improve patency of small-diameter vascular graft. Vascular grafts were tissue engineered with decellularized canine abdominal aortas and canine autologous bone marrow–derived cells. Prior to cell seeding onto decellularized graft matrices, bone marrow–derived cells were induced to differentiate into endothelial cells and smooth muscle cells. The cell-seeded vascular grafts were implanted into the abdominal aortas of bone marrow donor dogs. Before and after graft implantation, G-CSF was administered subcutaneously to the dogs (n = 3). The grafts implanted into the dogs not receiving G-CSF were used as controls (n = 3). Eight weeks after implantation, grafts in both groups showed regeneration of vascular tissues including endothelium and smooth muscle. Importantly, endothelium formation was more extensive in the G-CSF–treated grafts than in the control grafts, as assessed with reverse transcription polymerase chain reaction, western blot, and immunohistochemistry. In addition, intimal hyperplasia was significantly reduced in the G-CSF–treated grafts compared to the control grafts. This study suggests that G-CSF administration could be applied to improve patency of small-diameter tissue-engineered vascular grafts.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.30535/abstract
DOI
10.1002/jbm.a.30535
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
Yonsei Authors
Yoo, Kyung Jong(유경종) ORCID logo https://orcid.org/0000-0002-9858-140X
Hwang, Ki Chul(황기철)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/111068
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