Cited 22 times in
Apoptosis of macrophages induced by Trichomonas vaginalis through the phosphorylation of p38 mitogen-activated protein kinase that locates at downstream of mitochondria-dependent caspase activation
DC Field | Value | Language |
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dc.contributor.author | 최인홍 | - |
dc.date.accessioned | 2015-06-10T13:03:46Z | - |
dc.date.available | 2015-06-10T13:03:46Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 1357-2725 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/110938 | - |
dc.description.abstract | Trichomonas vaginalis, a flagellated protozoan parasite, is the causative organism of trichomoniasis. We have recently demonstrated that T. vaginalis induces apoptotic cell death via a Bcl-xL-dependent pathway in RAW264.7 macrophages. In this study, we attempted to characterize in detail the signaling cascades resulting in T. vaginalis-induced macrophage apoptosis, focusing particularly on mitochondrial changes and the role of p38 mitogen-activated protein kinase (p38 MAPK) activation. We found that T. vaginalis induced mitochondrial changes including the release of cytochrome c and the serial activation of caspases, leading to the activation of p38 MAPK in macrophages. These biochemical changes culminated in the apoptosis of the host cells. Caspase inhibitors induced a significant inhibition of T. vaginalis-induced nuclear damage, as well as the activation of p38 MAPK. Treatment with the p38 MAPK inhibitor, SB203580, or the overexpression of kinase-inactive p38 MAPK, induced an attenuation of T. vaginalis-induced apoptosis but not cytochrome c release, the activation of caspase-9 and caspase-3, or PARP cleavage. Furthermore, SB203580 treatment to human macrophages consistently blocked T. vaginalis-induced apoptosis. Collectively, our findings indicate that p38 MAPK signaling cascade is requisite to apoptosis of T. vaginalis-infected macrophage, and this apoptotic process occurs via the phosphorylation of p38 MAPK, which is located downstream of mitochondria-dependent caspase activation, conferring insight into the plausible molecular mechanism of T. vaginalis-immune evasion from macrophage attack. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 638~647 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis* | - |
dc.subject.MESH | Caspase 3 | - |
dc.subject.MESH | Caspase 9 | - |
dc.subject.MESH | Caspases/metabolism | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cytochromes c/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Macrophages/enzymology* | - |
dc.subject.MESH | Macrophages/parasitology | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mitochondria/enzymology* | - |
dc.subject.MESH | Mitochondria/parasitology | - |
dc.subject.MESH | Trichomonas Infections/enzymology* | - |
dc.subject.MESH | Trichomonas vaginalis/metabolism* | - |
dc.subject.MESH | p38 Mitogen-Activated Protein Kinases/metabolism* | - |
dc.title | Apoptosis of macrophages induced by Trichomonas vaginalis through the phosphorylation of p38 mitogen-activated protein kinase that locates at downstream of mitochondria-dependent caspase activation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Jae-Ho Chang | - |
dc.contributor.googleauthor | Soo-Ki Kim | - |
dc.contributor.googleauthor | In-Hong Choi | - |
dc.contributor.googleauthor | Sang-Kyou Lee | - |
dc.contributor.googleauthor | Tomohiro Morio | - |
dc.contributor.googleauthor | Eun-Ju Chang | - |
dc.identifier.doi | 10.1016/j.biocel.2005.11.005 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04167 | - |
dc.relation.journalcode | J01089 | - |
dc.identifier.eissn | 1878-5875 | - |
dc.identifier.pmid | 16360334 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S1357272505003833 | - |
dc.subject.keyword | Trichomonas vaginalis | - |
dc.subject.keyword | Apoptosis | - |
dc.subject.keyword | Macrophages | - |
dc.subject.keyword | p38 MAP kinase | - |
dc.contributor.alternativeName | Choi, In Hong | - |
dc.contributor.affiliatedAuthor | Choi, In Hong | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 38 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 638 | - |
dc.citation.endPage | 647 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, Vol.38(4) : 638-647, 2006 | - |
dc.identifier.rimsid | 55915 | - |
dc.type.rims | ART | - |
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