PPARγ activation induces CD36 expression and stimulates foam cell like changes in rVSMCs

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Authors: Hyun-Joung Lim ; Seahyoung Lee ; Kuy-Sook Lee ; Jin-Hee Park ; Yangsoo Jang ; Eun Jig Lee ; Hyun-Young Park

Citation: PROSTAGLANDINS & OTHER LIPID MEDIATORS, Vol.80(3-4) : 165-174, 2006

MeSH: ATP Binding Cassette Transporter 1 ; ATP-Binding Cassette Transporters/genetics ; Actins/genetics ; Adiponectin/genetics ; Animals ; CD36 Antigens/genetics ; CD36 Antigens/metabolism* ; Cells, Cultured ; Chromans/pharmacology ; Complement Factor D/genetics ; Fatty Acid-Binding Proteins/genetics ; Foam Cells/cytology ; Foam Cells/metabolism* ; Gene Expression/drug effects ; Hypoglycemic Agents/pharmacology ; Lipid Metabolism/drug effects ; Mice ; Muscle, Smooth, Vascular/cytology ; Muscle, Smooth, Vascular/drug effects ; Muscle, Smooth, Vascular/metabolism ; Myocytes, Smooth Muscle/cytology ; Myocytes, Smooth Muscle/drug effects ; Myocytes, Smooth Muscle/metabolism* ; PPAR alpha/genetics ; PPAR gamma/agonists ; PPAR gamma/genetics ; PPAR gamma/metabolism* ; Platelet-Derived Growth Factor/pharmacology ; Prostaglandin D2/analogs & derivatives ; Prostaglandin D2/pharmacology ; Rats ; Rats, Sprague-Dawley ; Thiazolidinediones/pharmacology ; Transfection ; Troglitazone ; Tropomyosin/genetics

Abstract: The purpose of the present study was to determine the role of peroxisome proliferator-activated receptor gamma (PPARγ) activation in smooth muscle cell (SMC) derived form cell formation. Wild and mutant type PPARγ were delivered by adenovirus then activated with troglitazone. The result of Oil Red O staining and FACS analysis showed that PPARγ activation induced lipid accumulation in rVSMCs. Furthermore, PPARγ activation reduced SMC marker genes such as α-actin while induced adipocyte differentiation marker genes and lipid metabolism-related genes as evidenced by RT-PCR and fluorescent immunocytochemistry. All these data demonstrate that PPARγ activation can drive foam cell like change in rVSMCs. Our results strongly suggest that PPARγ expression induces CD36 expression and adipocyte differentiation gene activation in the process of atherosclerosis and might be one of the crucial events in SMC derived foam cell formation.

Full Text: http://www.sciencedirect.com/science/article/pii/S1098882306000803

Appears in Collections:: 1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

Yonsei Authors: Lee, Eun Jig(이은직) https://orcid.org/0000-0002-9876-8370
Jang, Yang Soo(장양수) https://orcid.org/0000-0002-2169-3112

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