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Anti-cancer effect and structural characterization of endo-polysaccharide from cultivated mycelia of Inonotus obliquus

 Yong Ook Kim  ;  Hae Woong Park  ;  Jong Hoon Kim  ;  Jae Young Lee  ;  Seong Hoon Moon  ;  Chul Soo Shin 
 LIFE SCIENCES, Vol.79(1) : 72-80, 2006 
Journal Title
Issue Date
Animals ; Antibiotics, Antineoplastic/chemistry ; Antibiotics, Antineoplastic/isolation & purification ; Antibiotics, Antineoplastic/pharmacology* ; Antibody Formation/immunology ; B-Lymphocytes/immunology ; Basidiomycota/chemistry* ; Basidiomycota/genetics ; Body Weight/drug effects ; Cell Line ; Cell Survival/drug effects ; Chromatography, Gel ; Chromatography, Ion Exchange ; Culture Media ; Female ; Humans ; Macrophages/drug effects ; Macrophages/metabolism ; Magnetic Resonance Spectroscopy ; Mice ; Molecular Weight ; Mycelium/chemistry* ; Neoplasm Transplantation ; Nitric Oxide/biosynthesis ; Polysaccharides/chemistry ; Polysaccharides/isolation & purification ; Polysaccharides/pharmacology* ; Survival Analysis
Inonotus obliquus ; Endo-polysaccharide ; Anti-cancer activityImmuno-stimulating ; Chemical structure
The endo-polysaccharide extracted from mycelia of Inonotus obliquus (Pers.:Fr.) Pil. (Hymenochaetaceae) is a specific activator of B cells and macrophages. However, the in vivo anti-cancer effects and the chemical structure of the endo-polysaccharide are unknown. We purified the endo-polysaccharide, investigated its anti-cancer effects via in vitro and in vivo assays, and performed a structural characterization. The endo-polysaccharide was extracted from I. obliquus mycelia cultivated in a 300-l pilot fermenter, followed by hot water extraction and ethanol precipitation. Purification was achieved by DEAE-cellulose ion-exchange chromatography and gel-permeation chromatography. Chemical analysis revealed that the purified endo-polysaccharide is an α-linked fucoglucomannan with a molecular weight of approximately 1000 kDa. The anti-cancer activities of the endo-polysaccharide against various types of tumor cells were determined. No direct toxicity against either cancer or normal cells was observed. Intraperitoneal administration of the endo-polysaccharide significantly prolonged the survival rate of B16F10-implanted mice, resulting in a 4.07-fold increase in the survival rate at a dose of 30 mg/kg/day. After 60 days of feeding, approximately 67% of the initial number of mice survived with no tumor incidence based on macroscopic examination. These results indicate that the anti-cancer effect of endo-polysaccharide is not directly tumorcidal but rather is immuno-stimulating.
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1. College of Medicine (의과대학) > Dept. of Anesthesiology and Pain Medicine (마취통증의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jong Hoon(김종훈)
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