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vanA 유전자를 지닌 Vancomycin 내성 장구균의 VanB 표현형 발현 기전

Other Titles
 Mechanism of VanB Phenotype in Vancomycin-Resistant Enterococci carrying vanA gene 
Authors
 박일중  ;  이위교  ;  이혁민  ;  용동은  ;  이경원  ;  김의종  ;  정석훈  ;  박연준  ;  최태열  ;  어영  ;  신종희  ;  이종욱  ;  안지영  ;  이성희  ;  우건조 
Citation
 KOREAN JOURNAL OF LABORATORY MEDICINE , Vol.26(6) : 412-417, 2006 
Journal Title
KOREAN JOURNAL OF LABORATORY MEDICINE
ISSN
 1598-6535 
Issue Date
2006
Keywords
VRE ; VanB ; vanA ; vanS ; vanX ; vanY ; vanZ
Abstract
Background: Recently, vancomycin-resistant enterococci (VRE) with the vanA genotype that are susceptible to teicoplanin have been described in Japan, Taiwan, and Korea. The investigators suggested three point mutations in the putative sensor domain of vanS or impairment of accessory proteins VanY and VanZ as an explanation for the VanB phenotype-vanA genotype VRE. In this study, we analyzed Tn1546-like elements to determine the molecular mechanisms responsible for the impaired glycopeptide resistance of clinical VRE isolates with VanB phenotype-vanA genotype from Korea.

Methods: From 2001 to 2004, 28 clinical isolates of Enterococcus faecium with VanB phenotypevanA genotype were collected from 8 different university hospitals in diverse geographic areas in Korea. For structural analysis of Tn1546-like elements, PCR amplifications for internal regions of Tn1546 were performed. The purified PCR products were directly sequenced with an ABI Prism 3100 DNA sequencer.

Results: The sequence data of the vanS regulatory gene revealed that none of the isolates had any point mutations in this gene. All 28 isolates had a complete or incomplete deletion of vanY gene. Of these, 13 strains represented a complete deletion of vanZ, and 2 strains showed the deletion of nucleotides near the end point of vanX.

Conclusion: The mechanism of VanB phenotype-vanA genotype in VRE isolates from Korea is not point mutations of vanS but the rearrangements of vanX, vanY and vanZ.
Files in This Item:
T200601695.pdf Download
DOI
10.3343/kjlm.2006.26.6.412
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/110717
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