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Dual Effect of H2O2 on the Regulation of Cholecystokinin-induced Amylase Release in Rat Pancreatic Acinar Cells
DC Field | Value | Language |
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dc.contributor.author | 서정택 | - |
dc.date.accessioned | 2015-06-10T12:52:50Z | - |
dc.date.available | 2015-06-10T12:52:50Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 1226-7155 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/110614 | - |
dc.description.abstract | [H₂O₂], a member of reactive oxygen species (ROS), is known to be involved in the mediation of physiological functions in a variety of cell types. However, little has been known about the physiological role of H₂O₂in exocrine cells. Therefore, in the present study, the effect of H₂O₂on cholecystokinin (CCK)-evoked Cα²+ mobilization and amylase release was investigated in rat pancreatic acinar cells. Stimulation of the acinar cells with sulfated octapeptide form of CCK (CCK-8S) induced biphasic increase in amylase release. Addition of 30μM H₂O₂ enhanced amylase release caused by 10 pM CCK-8S, but inhibited the amylase release induced by CCK-8S at concentrations higher than 100 pM. An ROS scavenger, 10 μM Mn(III)tetrakis(4-benzoic acid)porphyrin chloride, increased amylase release caused by CCK-8S at concentrations higher than 100 pM, although lower concentrations of CCK-8S-induced amylase release was not affected. To examine whether the effect of H₂O₂on CCK-8S-induced amylase release was exerted via modulation of intracellular Cα²+ signaling, we measured the changes in intracellular Cα²+ concentration ([Cα²+]i) in fura-2 loaded acinar cells. Although 30 μM H₂O₂did not induce any increase in([Cα²+]i by itself, it increased the frequency and amplitude of([Cα²+]i oscillations caused by 10 pM CCK-8S. However, 30μM H₂O₂had little effect on 1 nM CCK-8S-induced increase in [Cα²+]i. ROS scavenger, 1 mM N-acetylcysteine, did not affect [Cα²+]i changes induced by 10 pM or 1 nM CCK-8S. Therefore, it was concluded that 30 μM H₂O₂ enhanced low concentration of CCK-8S-induced amylase release probably by increasing Cα²+]i oscillations while it inhibited high concentration of CCK-8S-induced amylase release. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 127~133 | - |
dc.relation.isPartOf | International Journal of Oral Biology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Dual Effect of H2O2 on the Regulation of Cholecystokinin-induced Amylase Release in Rat Pancreatic Acinar Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | An. Jeong-Mi | - |
dc.contributor.googleauthor | Rhie. Jin-Hak | - |
dc.contributor.googleauthor | Seo. Jeong-Taeg | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01905 | - |
dc.relation.journalcode | J01144 | - |
dc.subject.keyword | H2O2 | - |
dc.subject.keyword | cholecystokinin | - |
dc.subject.keyword | amylase release | - |
dc.subject.keyword | pancreatic acinar cells | - |
dc.contributor.alternativeName | Seo, Jeong Taeg | - |
dc.contributor.affiliatedAuthor | Seo, Jeong Taeg | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 31 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 127 | - |
dc.citation.endPage | 133 | - |
dc.identifier.bibliographicCitation | International Journal of Oral Biology, Vol.31(4) : 127-133, 2006 | - |
dc.identifier.rimsid | 57491 | - |
dc.type.rims | ART | - |
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