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Identification of α-enolase as an autoantigen associated with severe asthma

Authors
 Dong-Ho Nahm  ;  Kwang-Hoon Lee  ;  Jee-Young Shin  ;  Young-Min Ye  ;  Yup Kang  ;  Hae-Sim Park 
Citation
 JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, Vol.118(2) : 376-381, 2006 
Journal Title
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
ISSN
 0091-6749 
Issue Date
2006
MeSH
Adult ; Asthma/immunology* ; Autoantibodies/blood ; Autoantibodies/immunology ; Autoantigens/immunology* ; Biomarkers/blood ; Cell Line ; Epithelial Cells/immunology ; Female ; Humans ; Immunoglobulin G/blood ; Immunoglobulin G/immunology* ; Male ; Phosphopyruvate Hydratase/immunology* ; Respiratory Mucosa/immunology
Keywords
Asthma ; autoantibodies ; autoantigen ; airway epithelial cell
Abstract
BACKGROUND: Approximately 5% to 10% of patients with asthma have severe disease that is not effectively controlled by typical therapies. The existence of an autoantigen associated with severe asthma has been previously reported.
OBJECTIVE: We attempted to identify the autoantigen.
METHODS: Severe asthma was defined as patients having at least 1 severe asthmatic exacerbation requiring an emergency department visit or admission in the last year despite continuous typical therapies. Autoantibodies to airway epithelial cells (A549) were examined in sera from patients with severe asthma by immunoblot analysis.
RESULTS: IgG autoantibodies to the 52-kd airway epithelial cell antigen were detected in sera from 32 of 78 patients with severe asthma (41%), 9 of 83 patients with mild-to-moderate asthma (11%), and 2 of 58 healthy controls (3%; P < .001). The 52-kd autoantigen was identified as alpha-enolase by mass spectrometry analysis and confirmed by using recombinant human alpha-enolase protein. The detection of IgG autoantibodies to alpha-enolase was the most significant indicator for distinguishing severe asthma from mild-to-moderate asthma, even after adjusting for the effects of other clinical variables, including age, sex, atopy, and FEV(1) (adjusted odds ratio, 5.2; 95% CI, 2.1-12.9; P < .001).
CONCLUSION: The alpha-enolase was identified as an autoantigen associated with severe asthma. Further studies are needed to determine the significance of this autoantigen in severe asthma.
CLINICAL IMPLICATIONS: IgG autoantibodies to alpha-enolase could be a biological marker for severe asthma.
Full Text
http://www.sciencedirect.com/science/article/pii/S0091674906007494
DOI
10.1016/j.jaci.2006.04.002
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Kwang Hoon(이광훈)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/110400
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