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The behavioral effect of human mesenchymal stem cell transplantation in cold brain injured rats.

Authors
 Y. H. Cho  ;  H. S. Kim  ;  K. H. Lee  ;  Y. E. Lee  ;  J. W. Chang 
Citation
 Acta Neurochirurgica Supplements, Vol.99 : 125-132, 2006 
Journal Title
Acta Neurochirurgica Supplements
ISSN
 0065-1419 
Issue Date
2006
MeSH
Animals ; Behavior, Animal ; Bone Marrow Transplantation/pathology ; Brain Injuries/pathology ; Brain Injuries/therapy* ; Cerebral Cortex/pathology ; Humans ; Mesenchymal Stem Cell Transplantation* ; Models, Animal ; Motor Cortex/injuries ; Motor Cortex/pathology ; Rats ; Transplantation, Heterologous
Keywords
hMSCs ; transplantation ; behavior recovery ; traumatic cold injury ; immunostaining
Abstract
We investigated the effect of stereotaxically transplanted human mesenchymal stem cells (hMSCs) on behavioral change after traumatic cold brain injury in adult rats. Cortical lesions (n = 20) were induced by touching a metal stamp, cooled with liquid nitrogen, to the dura over the forelimb motor cortex of adult rats. The procedure produced a localized lesion, and the animals showed significant motor deficits. hMSCs were freshly isolated from human iliac bone and cultured in tissue culture flasks with 10 ml Dulbecco’s modified Eagle’s medium. The animals received hMSC grafts (3 ×105 hMSCs) 6 days after cold lesion (n = 10). All rats were sacrificed 3 or 7 weeks after cold injury, and immunohistochemical staining was performed on brain sections to identify donor hMSCs.
Neurological evaluations were performed with the forepaw adjusting step test and modified neurological scoring. Treatment with 3 ×105 hMSCs improved the rat’s neurological functions. We also found that the transplanted cells successfully migrated into the injured brain, preferentially localized around the injury site, and expressed the neuronal and astrocyte marker.
These data suggest that hMSCs may be a potential therapeutic tool for brain injuries.
Full Text
http://link.springer.com/chapter/10.1007/978-3-211-35205-2_24
DOI
10.1007/978-3-211-35205-2_24
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
Yonsei Authors
Chang, Jin Woo(장진우) ORCID logo https://orcid.org/0000-0002-2717-0101
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/110303
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