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Quantitative Assessment of hTERT mRNA Expression in Dysplastic Nodules of HBV-Related Hepatocarcinogenesis

Authors
 Bong-Kyeong Oh  ;  Young-Joo Kim  ;  Young Nyun Park  ;  Jinsub Choi  ;  Kyung Sik Kim  ;  Chanil Park 
Citation
 AMERICAN JOURNAL OF GASTROENTEROLOGY, Vol.101(4) : 831-838, 2006 
Journal Title
 AMERICAN JOURNAL OF GASTROENTEROLOGY 
ISSN
 0002-9270 
Issue Date
2006
MeSH
Adult ; Aged ; Carcinoma, Hepatocellular/metabolism* ; Carcinoma, Hepatocellular/pathology ; Carcinoma, Hepatocellular/virology ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism* ; Female ; Gene Dosage ; Hepatitis B/complications* ; Humans ; Liver/metabolism ; Liver/pathology ; Liver Diseases/metabolism* ; Liver Diseases/pathology ; Liver Neoplasms/metabolism* ; Liver Neoplasms/pathology ; Liver Neoplasms/virology ; Male ; Middle Aged ; Precancerous Conditions/diagnosis ; Precancerous Conditions/metabolism* ; Precancerous Conditions/pathology ; Proto-Oncogene Proteins c-myc/genetics ; RNA, Messenger/metabolism* ; Reverse Transcriptase Polymerase Chain Reaction ; Telomerase/genetics ; Telomerase/metabolism*
Abstract
BACKGROUND: Telomerase reverse transcriptase (hTERT) is the rate-limiting determinant of telomerase, which is critical for carcinogenesis. Dysplastic nodules (DNs) appear to be preneoplastic lesions of hepatocellular carcinomas (HCCs). In this study, in order to characterize DNs, hTERT mRNA, hTERT gene dosage, and mRNA for c-myc, a transcriptional activator of hTERT were studied in human multi-step hepatocarcinogenesis. METHODS: Fifty four hepatic nodules including 5 large regenerative nodules, 14 low-grade DNs, 7 high-grade DNs, 11 DNs with HCC foci and 17 HCCs, 23 livers with chronic hepatitis/cirrhosis, and 6 normal livers were examined. Transcript levels were measured by real-time quantitative RT-PCR and gene dosages by real-time PCR and Southern blotting. RESULTS: The hTERT mRNA levels increased with the progression of hepatocarcinogenesis, and a significant induction in the transition between low- and high-grade DNs was seen. Most high-grade DNs strongly expressed hTERT mRNA at levels similar to those of HCCs. Twenty-one percent of low-grade DNs had high levels of hTERT mRNA, up to those of high-grade DNs and there was no difference in the pathological features between low-grade DNs with and without increased hTERT mRNA levels. No correlation was found between hTERT mRNA levels, hTERT gene dosage, and c-myc mRNA levels. CONCLUSIONS: These results suggest that the induction of hTERT mRNA is an important early event and that its measurement by real-time quantitative RT-PCR is a useful tool to detect premalignant/malignant tendencies in hepatic nodules. However, hTERT gene dosage and c-myc expression are not the main mechanisms regulating hTERT expression in hepatocarcinogenesis.
Full Text
http://www.nature.com/ajg/journal/v101/n4/full/ajg2006157a.html
DOI
10.1111/j.1572-0241.2006.00532.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Sik(김경식) ORCID logo https://orcid.org/0000-0001-9498-284X
Park, Young Nyun(박영년) ORCID logo https://orcid.org/0000-0003-0357-7967
Park, Chan Il(박찬일)
Choi, Jin Sub(최진섭)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/110261
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