459 450

Cited 15 times in

Proteomics Analysis of Apoptosis-regulating Proteins in Tissues with Different Radiosensitivity

Authors
 Jeung Hee AN  ;  Jin Sil SEONG 
Citation
 JOURNAL OF RADIATION RESEARCH, Vol.47(2) : 147-155, 2006 
Journal Title
JOURNAL OF RADIATION RESEARCH
ISSN
 0449-3060 
Issue Date
2006
MeSH
Animals ; Apoptosis/physiology* ; Apoptosis/radiation effects ; Apoptosis Regulatory Proteins/metabolism* ; Gene Expression Regulation/physiology* ; Gene Expression Regulation/radiation effects ; Liver/metabolism* ; Liver/radiation effects ; Mice ; Mice, Inbred C3H ; Organ Specificity ; Proteome/metabolism* ; Radiation Dosage ; Radiation Tolerance/physiology* ; Reactive Oxygen Species/metabolism ; Spleen/metabolism* ; Spleen/radiation effects ; Tissue Distribution ; Whole-Body Irradiation
Abstract
The aim of this study was to identify of radiosusceptibility proteins in tissues with different radiosensitivity. C3H/HeJ mice were exposed to 10 Gy. The tissues were processed for proteins extraction and were analyzed by 2-dimensional electrophoresis. The proteins were identified by matrix-assisted laser desorption ionizing time-of-flight mass spectrometry and validated by immunohistochemical staining and Western blotting. The peaks of apoptosis levels were 35.3 ± 1.7% and 0.6 ± 0.2% in the spleen and the liver, respectively, after ionizing radiation. Analysis of liver tissue showed that the expression level of ROS related proteins such as cytochrome c, glutathione S transferase, NADH dehydrogenase and peroxiredoxin VI increased after radiation. The expression level of cytochrome c increased to 3-fold after ionizing radiation in both tissues. However in spleen tissue, the expression level of various kinds of apoptosis regulating proteins increased after radiation. These involved iodothyronine, CD 59A glycoprotein precursor, fas antigen and tumor necrosis factor -inducible protein TSG-6nprecursor after radiation. The difference in the apoptosis index between the liver and spleen tissues is closely associated with the expression of various kinds of apoptosis-related proteins. The result suggests that the expression of apoptosis-related protein and redox proteins play important roles in this radiosusceptibility.
Files in This Item:
T200601118.pdf Download
DOI
10.1269/jrr.47.147
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
Yonsei Authors
Seong, Jin Sil(성진실) ORCID logo https://orcid.org/0000-0003-1794-5951
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/110083
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links