Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

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Authors: Eui‐Chul Park ; Jong‐Bok Yoon ; Jin‐Sil Seong ; Kyoung‐Soo Choi ; Eung‐Sik Kong ; Yun‐Jeong Kim ; Young‐Mee Park ; Eun‐Mi Park

MeSH: Animals ; Electrophoresis, Gel, Two-Dimensional ; Gamma Rays ; Glutathione/metabolism ; Lipid Peroxidation/radiation effects ; Liver/metabolism ; Liver/radiation effects* ; Male ; Proteins/analysis ; Proteome/analysis* ; Proteomics/methods* ; Rats ; Rats, Sprague-Dawley ; Reactive Oxygen Species/metabolism ; Spectrometry, Mass, Electrospray Ionization ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Thiobarbituric Acid Reactive Substances/metabolism ; Up-Regulation/radiation effects

Abstract: Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI‐TOF‐MS and ESI‐MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.

Full Text: http://www.tandfonline.com/doi/abs/10.1080/10826060500388470#.U6e81qizIyo

Appears in Collections:: 1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers

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