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Generation of nitric oxide in the opossum lower esophageal sphincter during physiological experimentation

 Se-joon Lee  ;  Hyojin Park  ;  Jin Hyuck Chang  ;  Jeffrey L. Conklin 
 YONSEI MEDICAL JOURNAL, Vol.47(2) : 223-229, 2006 
Journal Title
Issue Date
Animals ; Antioxidants/chemistry ; Antioxidants/metabolism ; Blotting, Western ; Catalase/metabolism ; Esophageal Sphincter, Lower/anatomy & histology* ; Esophageal Sphincter, Lower/metabolism ; Esophageal Sphincter, Upper/anatomy & histology* ; Esophageal Sphincter, Upper/metabolism ; Female ; Lipopolysaccharides/chemistry ; Lipopolysaccharides/metabolism ; Luminescence ; Male ; Muscles/metabolism ; Nitric Oxide/chemistry* ; Nitric Oxide/metabolism ; Nitric Oxide Synthase Type II/metabolism ; Opossums ; Superoxide Dismutase/metabolism ; Time Factors ; Tyrosine/analogs & derivatives ; Tyrosine/chemistry
Nitric oxide ; lower esophageal sphincter
Lipopolysaccharide (LPS), given in vivo, modulates opossum esophageal motor functions by inducing the inducible nitric oxide synthase (iNOS), which increases nitric oxide (NO) production. Superoxide, a NO scavenger, is generated during this endotoxemia. Superoxide is cleared by superoxide dismutase (SOD) and catalase (CAT) to protect the physiological function of NO. This study examined whether lower esophageal sphincter (LES) motility, NO release, and iNOS and nitrotyrosine accumulation in the LES are affected by LPS in vitro. Muscle strips from the opossum LES were placed in tissue baths containing oxygenated Krebs buffer. NO release was measured with a chemiluminescence NOx analyzer, and Western blots were performed to analyze iNOS and nitrotyrosine production. The percent change in resting LES tone after a 6-hour exposure to LPS was significantly increased compared to pretreatment values. The percent LES relaxation upon electrical stimulation was significantly decreased in the control group at 6 hours, indicating that the LPS treatment had an effect. The NO concentration in the tissue bath of LPS-treated muscle without nerve stimulation was significantly less than that of LPS treatment combined with SOD/CAT or SOD/CAT alone. iNOS and nitrotyrosine were detectable and increased over time in the LES muscle of both the control and LPS-treated groups. Antioxidant enzymes may play a role in regulating NO-mediated neuromuscular functions in the LES.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Park, Hyo Jin(박효진) ORCID logo https://orcid.org/0000-0003-4814-8330
Lee, Se Joon(이세준) ORCID logo https://orcid.org/0000-0002-2695-2670
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