Cited 55 times in
KAI1/CD82 suppresses tumor invasion by MMP9 inactivation via TIMP1 up-regulation in the H1299 human lung carcinoma cell line
DC Field | Value | Language |
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dc.contributor.author | 이운규 | - |
dc.date.accessioned | 2015-06-10T12:26:06Z | - |
dc.date.available | 2015-06-10T12:26:06Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/109795 | - |
dc.description.abstract | We conducted a study on the mechanism of KAI1/CD82-mediated suppression of tumor invasiveness and metastasis, and examined its effect on MMP-9 activity and the TIMP1 levels in H1299 human non-small cell lung carcinoma cells. The H1299 human lung carcinoma cells were transfected with pcDNA3.1-CD82 and stable transfectant clones that had a high KAI1/CD82 expression were obtained. We performed Western blot analysis, cell invasion assay, gelatin zymography, and RT-PCR to assess the KAI1/CD82 expression and tumor invasiveness, the MMP-9 activity, the MMP-9 mRNA and protein levels, and the TIMP1 levels in the H1299/CD82 transfectant cells and compared the results with those of the control groups. The H1299/CD82 transfectants exhibited significant suppression of cell invasion, reduced MMP9 enzyme activity, elevated MMP9 mRNA and MMP-9 protein levels, and elevated TIMP1 levels. It may be postulated that KAI1/CD82 over-expression in the H1299 non-small cell lung carcinoma cells suppresses the tumor invasiveness and metastatic potential by inducing MMP9 inactivation via the up-regulation of TIMP1. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 655~661 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Carcinoma/enzymology | - |
dc.subject.MESH | Carcinoma/metabolism | - |
dc.subject.MESH | Carcinoma/pathology* | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Kangai-1 Protein/biosynthesis | - |
dc.subject.MESH | Kangai-1 Protein/genetics | - |
dc.subject.MESH | Kangai-1 Protein/physiology* | - |
dc.subject.MESH | Lung Neoplasms/enzymology* | - |
dc.subject.MESH | Lung Neoplasms/metabolism | - |
dc.subject.MESH | Lung Neoplasms/pathology* | - |
dc.subject.MESH | Matrix Metalloproteinase 9/genetics | - |
dc.subject.MESH | Matrix Metalloproteinase 9/metabolism* | - |
dc.subject.MESH | Matrix Metalloproteinase Inhibitors* | - |
dc.subject.MESH | Neoplasm Invasiveness/pathology | - |
dc.subject.MESH | Neoplasm Invasiveness/prevention & control | - |
dc.subject.MESH | RNA, Messenger/metabolism | - |
dc.subject.MESH | Tissue Inhibitor of Metalloproteinase-1/genetics | - |
dc.subject.MESH | Tissue Inhibitor of Metalloproteinase-1/physiology* | - |
dc.subject.MESH | Transfection | - |
dc.subject.MESH | Up-Regulation/physiology* | - |
dc.title | KAI1/CD82 suppresses tumor invasion by MMP9 inactivation via TIMP1 up-regulation in the H1299 human lung carcinoma cell line | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | Bo Keun Jee | - |
dc.contributor.googleauthor | Koung Min Park | - |
dc.contributor.googleauthor | Sibin Surendran | - |
dc.contributor.googleauthor | Woon Kyu Lee | - |
dc.contributor.googleauthor | Chang Whan Han | - |
dc.contributor.googleauthor | Yong Sik Kim | - |
dc.contributor.googleauthor | Young Lim | - |
dc.identifier.doi | 10.1016/j.bbrc.2006.01.153 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02996 | - |
dc.relation.journalcode | J00281 | - |
dc.identifier.eissn | 1090-2104 | - |
dc.identifier.pmid | 16488391 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0006291X06002439 | - |
dc.subject.keyword | H1299 non-small cell lung carcinoma cells | - |
dc.subject.keyword | KAI1/CD82 | - |
dc.subject.keyword | MMP9 | - |
dc.subject.keyword | TIMP1 | - |
dc.subject.keyword | Tumor invasiveness | - |
dc.contributor.alternativeName | Lee, Woon Kyu | - |
dc.contributor.affiliatedAuthor | Lee, Woon Kyu | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 342 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 655 | - |
dc.citation.endPage | 661 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.342(2) : 655-661, 2006 | - |
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