Cited 9 times in
Evidence for Cyclooxygenase-2 Association with Caveolin-3 in Primary Cultured Rat Chondrocytes
DC Field | Value | Language |
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dc.contributor.author | 이운규 | - |
dc.date.accessioned | 2015-06-10T12:25:58Z | - |
dc.date.available | 2015-06-10T12:25:58Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 1011-8934 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/109791 | - |
dc.description.abstract | The purpose of this study was to demonstrate the cellular localization of cyclooxygenase-2 (COX-2) and caveolin-3 (Cav-3) in primarily cultured rat chondrocytes. In normal rat chondrocytes, we observed relatively high levels of Cav-3 and a very low level of COX-2 mRNA and protein. Upon treating the chondrocytes with 5 µM of CdCl2 (Cd) for 6 hr, the expressions of COX-2 mRNA and protein were increased with the decreased Cav-3 mRNA and protein expressions. The detergent insoluble caveolae-rich membranous fractions that were isolated from the rat chondrocytes and treated with Cd contained the both proteins of both COX-2 and Cav-3 in a same fraction. The immuno-precipitation experiments showed complex formation between the COX-2 and Cav-3 in the rat chondrocytes. Purified COX-2 with glutathione S-transferase-fused COX-2 also showed complex formation with Cav-3. Confocal and electron microscopy also demonstrated the co-localization of COX-2 and Cav-3 in the plasma membrane. The results from our current study show that COX-2 and Cav-3 are co-localized in the caveolae of the plasma membrane, and they form a protein-protein complex. The co-localization of COX-2 with Cav-3 in the caveolae suggests that the caveolins might play an important role for regulating the function of COX-2. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 100~106 | - |
dc.relation.isPartOf | JOURNAL OF KOREAN MEDICAL SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Animals, Newborn | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Cadmium Chloride/pharmacology | - |
dc.subject.MESH | Caveolae/drug effects | - |
dc.subject.MESH | Caveolae/metabolism | - |
dc.subject.MESH | Caveolae/ultrastructure | - |
dc.subject.MESH | Caveolin 3/genetics* | - |
dc.subject.MESH | Caveolin 3/metabolism | - |
dc.subject.MESH | Cell Membrane/drug effects | - |
dc.subject.MESH | Cell Membrane/metabolism | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Chondrocytes/cytology | - |
dc.subject.MESH | Chondrocytes/drug effects | - |
dc.subject.MESH | Chondrocytes/metabolism* | - |
dc.subject.MESH | Cyclooxygenase 2/genetics* | - |
dc.subject.MESH | Cyclooxygenase 2/metabolism | - |
dc.subject.MESH | Gene Expression | - |
dc.subject.MESH | Immunoprecipitation | - |
dc.subject.MESH | Microscopy, Confocal | - |
dc.subject.MESH | Microscopy, Electron | - |
dc.subject.MESH | RNA, Messenger/genetics | - |
dc.subject.MESH | RNA, Messenger/metabolism | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.title | Evidence for Cyclooxygenase-2 Association with Caveolin-3 in Primary Cultured Rat Chondrocytes | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | Jin-Oh Kwak | - |
dc.contributor.googleauthor | Woon Kyu Lee | - |
dc.contributor.googleauthor | Hyun-Woo Kim | - |
dc.contributor.googleauthor | Sun-Mi Jung | - |
dc.contributor.googleauthor | Kwang-Jin Oh | - |
dc.contributor.googleauthor | Sang-Yong Jung | - |
dc.contributor.googleauthor | Yang Hoon Huh | - |
dc.contributor.googleauthor | Seok Ho Cha | - |
dc.identifier.doi | 10.3346/jkms.2006.21.1.100 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02996 | - |
dc.relation.journalcode | J01517 | - |
dc.identifier.eissn | 1598-6357 | - |
dc.identifier.pmid | 16479074 | - |
dc.subject.keyword | Chondrocytes | - |
dc.subject.keyword | Cyclooxygenase 2 | - |
dc.subject.keyword | Caveolins | - |
dc.subject.keyword | Microscopy, Confocal | - |
dc.subject.keyword | Microscopy, Electron | - |
dc.contributor.alternativeName | Lee, Woon Kyu | - |
dc.contributor.affiliatedAuthor | Lee, Woon Kyu | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 21 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 100 | - |
dc.citation.endPage | 106 | - |
dc.identifier.bibliographicCitation | JOURNAL OF KOREAN MEDICAL SCIENCE, Vol.21(1) : 100-106, 2006 | - |
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