Helicobacter pylori 감염에서 새로운 저용량 13C-요소캡슐과 기존의 정제형 13C-요소제제를 이용한 요소호기검사간 비교

Abstract

Background : The urea breath test (UBT) is regarded as a highly reliable, noninvasive tool for diagnosing Helicobacter pylori infection. We compared a recently developed low-dose 38 mg 13C-urea capsule, which is able to eliminate oral urease effects and does not require positional changes during the test, with the conventionally used 100 mg 13C-urea tablet method.

Methods : Thirty-nine volunteers were tested under informed consent with both 13C-UBT methods, Helifinder and UBiT-IR300, with a minimum 2-week washout period. The pre-ingestion and 20- minute post-ingestion breath samples were analyzed with an isotope ratio mass spectrometer for Helifinder, and a nondispersive isotope-selective infrared spectrophotometer for UBiT samples.

Results : Helifinder method showed excellent agreement with UBiT among 19 positive and 20 negative cases (weighted kappa value, 1.0). Helifinder results (y) showed good agreement but with a proportional bias compared to UBiT results (x) by Passing and Bablok method (y=0.551x-0.255, r=0.74, P<0.0001).

Conclusion : Since the low-dose 38 mg 13C-urea capsule (Helifinder) test, which is more convenient and economic, showed comparable results with the conventional UBiT method, it can be used as an alternative for the diagnosis of H. pylori infection.