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Heterogeneous nuclear ribonuclear protein C is increased in the celecoxib-induced growth inhibition of human oral squamous cell carcinoma

Authors
 Eun Ju Lee  ;  Seong Hwan Kim  ;  Young Eun Kwark  ;  Jin Kim 
Citation
 EXPERIMENTAL AND MOLECULAR MEDICINE, Vol.38(3) : 203-209, 2006 
Journal Title
 EXPERIMENTAL AND MOLECULAR MEDICINE 
ISSN
 1226-3613 
Issue Date
2006
MeSH
Actins/metabolism ; Aged ; Carcinoma, Squamous Cell/metabolism ; Carcinoma, Squamous Cell/pathology ; Celecoxib ; Cell Cycle/drug effects ; Cell Line, Tumor ; Cell Proliferation/drug effects* ; Cell Survival/drug effects ; Cyclin-Dependent Kinase Inhibitor p27/analysis ; Cyclin-Dependent Kinase Inhibitor p27/metabolism ; Cyclooxygenase 2/metabolism ; Cyclooxygenase Inhibitors/pharmacology ; Electrophoresis, Gel, Two-Dimensional ; Heterogeneous-Nuclear Ribonucleoprotein Group C/analysis ; Heterogeneous-Nuclear Ribonucleoprotein Group C/metabolism* ; Humans ; Immunoblotting ; Male ; Proteomics/methods ; Pyrazoles/pharmacology* ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Sulfonamides/pharmacology* ; Tongue Neoplasms/metabolism ; Tongue Neoplasms/pathology ; Tumor Cells, Cultured
Keywords
carcinoma, squamous cell ; celecoxib ; cell cycle ; cyclin-dependent kinase inhibitor p27 ; cyclooxygenase 2 inhibitors ; heterogeneous-nuclear ribonucleoprotein C
Abstract
Celecoxib is a selective inhibitor of cyclooxygenase-2 (COX-2) that is a critical factor in carcinogenesis, but precise mechanism of its action remains to be elucidated. Here we evaluated the inhibitory effect of celecoxib on cell growth of human oral squamous cell carcinoma (OSCC) YD-10B, which was established to be used as in vitro OSCC model, and identified celecoxib-regulated protein by proteomics techniques. Celecoxib (IC50=37 µM) inhibited the growth of YD-10B cells with the decrease of COX-2 protein expression. Its inhibition could be linked in the arrest of G1 phase with increased levels of p(27)protein, a specific CDK inhibitor. Using proteomics, the 10- to 20-fold increase of heterogeneous nuclear ribonuclear protein C (hnRNP C), which has been suggested to be related with the translation of p(27)mRNA, was observed in celecoxib-treated YD-10B cells. In summary, celecoxib has a potential to induce the protein expression of hnRNP C and its increase subsequently induce the translation of p(27)mRNA, which trigger the inhibition of cell growth via p(27)-regulated cell cycle arrest in YD-10B cells. In addition, YD-10B cells could be useful to study the pathological mechanism of OSCC.
Files in This Item:
T200600784.pdf Download
DOI
10.1038/emm.2006.25
Appears in Collections:
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jin(김진)
Lee, Eun Ju(이은주)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/109704
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