Cited 41 times in
Critical Role of Phospholipase Cγ1 in the Generation of H2O2-evoked [Ca2+]i Oscillations in Cultured Rat Cortical Astrocytes
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 문석준 | - |
dc.contributor.author | 서정택 | - |
dc.contributor.author | 신동민 | - |
dc.contributor.author | 이승일 | - |
dc.date.accessioned | 2015-06-10T12:10:58Z | - |
dc.date.available | 2015-06-10T12:10:58Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/109338 | - |
dc.description.abstract | Reactive oxygen species, such as the superoxide anion, H2O2, and the hydroxyl radical, have been considered as cytotoxic by-products of cellular metabolism. However, recent studies have provided evidence that H2O2 serves as a signaling molecule modulating various physiological functions. Here we investigated the effect of H2O2 on the regulation of intracellular Ca2+ signaling in rat cortical astrocytes. H2O2 triggered the generation of oscillations of intracellular Ca2+ concentration ([Ca2+]i) in a concentration-dependent manner over the range 10-100 microM. The H2O2-induced [Ca2+]i oscillations persisted in the absence of extracellular Ca2+ and were prevented by depletion of intracellular Ca2+ stores with thapsigargin. The H2O2-induced [Ca2+]i oscillations were not inhibited by pretreatment with ryanodine but were prevented by 2-aminoethoxydiphenyl borate and caffeine, known antagonists of inositol 1,4,5-trisphosphate receptors. H2O2 activated phospholipase C (PLC) gamma1 in a dose-dependent manner, and U73122, an inhibitor of PLC, completely abolished the H2O2-induced [Ca2+]i oscillations. In addition, RNA interference against PLCgamma1 and the expression of the inositol 1,4,5-trisphosphate-sequestering "sponge" prevented the generation of [Ca2+]i oscillations. H2O2-induced [Ca2+]i oscillations and PLC1 phosphorylation were inhibited by pretreatment with dithiothreitol, a sulfhydryl-reducing agent. Finally, epidermal growth factor induced H2O2 production, PLCgamma1 activation, and [Ca2+]i increases, which were attenuated by N-acetylcysteine and diphenyleneiodonium and by the overexpression of peroxiredoxin type II. Therefore, we conclude that low concentrations of exogenously applied H2O2 generate [Ca2+]i oscillations by activating PLCgamma1 through sulfhydryl oxidation-dependent mechanisms. Furthermore, we show that this mechanism underlies the modulatory effect of endogenously produced H2O2 on epidermal growth factor-induced Ca2+ signaling in rat cortical astrocytes. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 13057~13067 | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Astrocytes/drug effects* | - |
dc.subject.MESH | Astrocytes/metabolism* | - |
dc.subject.MESH | Calcium/metabolism* | - |
dc.subject.MESH | Calcium Signaling/drug effects* | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Cerebral Cortex/cytology* | - |
dc.subject.MESH | Dose-Response Relationship, Drug | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Epidermal Growth Factor | - |
dc.subject.MESH | Estrenes | - |
dc.subject.MESH | Hydrogen Peroxide/pharmacology* | - |
dc.subject.MESH | Inositol 1,4,5-Trisphosphate/metabolism | - |
dc.subject.MESH | Phospholipase C gamma/antagonists & inhibitors | - |
dc.subject.MESH | Phospholipase C gamma/metabolism* | - |
dc.subject.MESH | Pyrrolidinones | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Wistar | - |
dc.subject.MESH | Thapsigargin | - |
dc.title | Critical Role of Phospholipase Cγ1 in the Generation of H2O2-evoked [Ca2+]i Oscillations in Cultured Rat Cortical Astrocytes | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Jeong Hee Hong | - |
dc.contributor.googleauthor | Seok Jun Moon | - |
dc.contributor.googleauthor | Hae Mi Byun | - |
dc.contributor.googleauthor | Min Seuk Kim | - |
dc.contributor.googleauthor | Hae Jo | - |
dc.contributor.googleauthor | Yun Soo Bae | - |
dc.contributor.googleauthor | Syng-Ill Lee | - |
dc.contributor.googleauthor | Martin D. Bootman | - |
dc.contributor.googleauthor | H. Llewelyn Roderick | - |
dc.contributor.googleauthor | Dong Min Shin | - |
dc.contributor.googleauthor | Jeong Taeg Seo | - |
dc.identifier.doi | 10.1074/jbc.M601726200 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01358 | - |
dc.contributor.localId | A01905 | - |
dc.contributor.localId | A02091 | - |
dc.contributor.localId | A02924 | - |
dc.relation.journalcode | J01258 | - |
dc.identifier.eissn | 1083-351X | - |
dc.identifier.pmid | 16543237 | - |
dc.contributor.alternativeName | Moon, Seok Jun | - |
dc.contributor.alternativeName | Seo, Jeong Taeg | - |
dc.contributor.alternativeName | Shin, Dong Min | - |
dc.contributor.alternativeName | Lee, Syng Ill | - |
dc.contributor.affiliatedAuthor | Moon, Seok Jun | - |
dc.contributor.affiliatedAuthor | Seo, Jeong Taeg | - |
dc.contributor.affiliatedAuthor | Shin, Dong Min | - |
dc.contributor.affiliatedAuthor | Lee, Syng Ill | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 281 | - |
dc.citation.number | 19 | - |
dc.citation.startPage | 13057 | - |
dc.citation.endPage | 13067 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, Vol.281(19) : 13057-13067, 2006 | - |
dc.identifier.rimsid | 52290 | - |
dc.type.rims | ART | - |
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