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Trichomonas vaginalis promotes apoptosis of human neutrophils by activating caspase-3 and reducing Mcl-1 expression

Authors
 J. H. KANG  ;  H. O. SONG  ;  J. S. RYU  ;  M. H. SHIN  ;  J. M. KIM  ;  Y. S. CHO  ;  J. F. ALDERETE  ;  M. H. AHN  ;  D. Y. MIN 
Citation
 PARASITE IMMUNOLOGY, Vol.28(9) : 439-446, 2006 
Journal Title
 PARASITE IMMUNOLOGY 
ISSN
 0141-9838 
Issue Date
2006
MeSH
Animals ; Apoptosis/immunology* ; Caspase 3 ; Caspases/metabolism* ; Enzyme Activation ; Female ; Humans ; Myeloid Cell Leukemia Sequence 1 Protein ; Neoplasm Proteins/biosynthesis* ; Neoplasm Proteins/genetics ; Neutrophils/enzymology ; Neutrophils/immunology* ; Neutrophils/parasitology* ; Proto-Oncogene Proteins c-bcl-2/biosynthesis* ; Proto-Oncogene Proteins c-bcl-2/genetics ; Trichomonas Vaginitis/parasitology ; Trichomonas vaginalis/immunology* ; Trichomonas vaginalis/isolation & purification
Keywords
Trichomonas vaginalis ; neutrophil ; apoptosis ; caspase‐3 ; Mcl‐1
Abstract
Neutrophils are the predominant inflammatory cells found in the vaginal discharge of patients with Trichomonas vaginalis infection. However, it is not known whether neutrophil apoptosis is induced by live T. vaginalis. Therefore, we examined whether T. vaginalis can influence neutrophil apoptosis, and also whether caspase-3 and the Bcl-2 family members are involved in the apoptosis. Thus, human neutrophils were incubated with live T. vaginalis and neutrophil apoptosis was evaluated by Giemsa, annexin V-PI, and DiOC6 stainings. The neutrophil apoptosis was significantly higher in those incubated with T. vaginalis than in the control group. When trichomonads were pre-treated with mAb to AP65 (adhesin protein), or when trophozoites were separated from neutrophils using a Transwell chamber, neutrophil apoptosis was significantly reduced. The activation of caspase-3 was evident in neutrophils undergoing spontaneous apoptosis but was markedly enhanced during T. vaginalis-induced apoptosis. Moreover, the inhibition of caspase-3 effectively reduced T. vaginalis-induced apoptosis. Trichomonad-induced apoptosis was also associated with reduced expression of the neutrophil anti-apoptotic protein, Mcl-1. These results indicate that T. vaginalis alters Mcl-1 expression and caspase-3 activation, thereby inducing apoptosis of human neutrophils.
Files in This Item:
T200600473.pdf Download
DOI
10.1111/j.1365-3024.2006.00884.x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Shin, Myeong Heon(신명헌) ORCID logo https://orcid.org/0000-0001-8207-6110
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/109333
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