4 287

Cited 24 times in

Vaccination with DNA encoding cysteine proteinase confers protective immune response to rats infected with Clonorchis sinensis

Authors
 Ji-Sook Lee  ;  In Sik Kim  ;  Woon-Mok Sohn  ;  Jongweon Lee  ;  Tai-Soon Yong 
Citation
 VACCINE, Vol.24(13) : 2358-2366, 2006 
Journal Title
 VACCINE 
ISSN
 0264-410X 
Issue Date
2006
MeSH
Amino Acid Sequence ; Animals ; Antibodies, Helminth/blood ; Antibody-Dependent Cell Cytotoxicity ; Clonorchiasis/prevention & control* ; Clonorchis sinensis/immunology* ; Cross Reactions ; Cysteine Endopeptidases/genetics* ; Cysteine Endopeptidases/immunology ; Female ; Immunoglobulin G/blood ; Interferon-gamma/biosynthesis ; Molecular Sequence Data ; Rats ; Rats, Sprague-Dawley ; Sequence Analysis, DNA ; Vaccination ; Vaccines, DNA/immunology*
Keywords
DNA vaccine ; Cysteine proteinase ; Th-1
Abstract
Cysteine proteinases of C. sinensis are important virulence factors that induce pathological changes associated with larval migration and localized biliary epithelial destruction. This study investigated the immunogenicity and protective efficacy of a DNA vaccine encoding Clonorchis sinensis cysteine proteinase (CsCP). The CsCP cDNA sequence displays significant homology to the mammalian or trematode cathepsin L. Plasmid DNA carrying the CsCP gene (pcDNA3.1-CsCP) was injected into Sprague-Dawley (SD) rats intradermally. Animals injected with pcDNA3.1-CsCP developed CsCP-specific antibodies, which exhibited an IgG2a dominance in sera. In addition, the DNA vaccine elicited the production of IFN-gamma, but not IL-4 in splenocytes, suggesting the induction of a typical Th-1 dominated immune response in rats. The pcDNA3.1-CsCP induced a significant level of protection (31.5%, p<0.05) in SD rats challenged with C. sinensis metacercariae. These results indicate that pcDNA3.1-CsCP induces both humoral and cellular immune responses. The CsCP gene may be a good candidate for use in future studies of vaccination against clonorchiasis.
Full Text
http://www.sciencedirect.com/science/article/pii/S0264410X0501203X
DOI
10.1016/j.vaccine.2005.11.062
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/109322
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse