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Suppression of human selenium-binding protein 1 is a late event in colorectal carcinogenesis and is associated with poor survival

 Hyunki Kim  ;  Hyun Ju Kang  ;  Kwon Tae You  ;  Se Hoon Kim  ;  Kang Young Lee  ;  Tae Il Kim  ;  Chul Kim  ;  Si Young Song  ;  Hye-Jung Kim  ;  Cheolju Lee  ;  Hoguen Kim 
 PROTEOMICS, Vol.6(11) : 3466-3476, 2006 
Journal Title
Issue Date
Adenoma/diagnosis ; Adenoma/metabolism* ; Adenoma/pathology ; Biomarkers, Tumor/metabolism ; Carcinoma/diagnosis ; Carcinoma/metabolism* ; Carcinoma/pathology ; Colon/metabolism ; Colon/pathology ; Colorectal Neoplasms/diagnosis ; Colorectal Neoplasms/metabolism* ; Colorectal Neoplasms/pathology ; Down-Regulation ; Electrophoresis, Gel, Two-Dimensional ; Humans ; Intestinal Mucosa/metabolism* ; Intestinal Mucosa/pathology ; Prognosis ; Proteome/metabolism* ; Selenium-Binding Proteins/metabolism* ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Tissue Array Analysis
Biomarker ; Colorectal carcinoma ; Prognosis ; Selenium‐binding protein 1
The purpose of this study was to analyze altered protein expression in cancer tissues and determine its relationship to prognosis in colorectal carcinomas. We performed proteomic expression analysis on 14 colorectal carcinomas and matched nontumorous colonic mucosa by 2-DE and MALDI-TOF-MS. Comparative analysis of the respective spot patterns on 2-DE showed 14 spots that were markedly changed in the colorectal carcinomas. Among them, selenium-binding protein 1 (SELENBP1) was markedly decreased in 12 (85%) carcinomas. The reduced expression of SELENBP1 was further supported by Western blot analysis and immunohistochemistry. Suppression of SELENBP1 was further analyzed in another eight-paired adenomas and carcinomas from the same patients using Western blot analysis and immunohistochemistry, and revealed that one adenoma and seven carcinomas exhibited markedly reduced SELENBP1 expression. Patients with low levels of SELENBP1 expression had significantly lower overall survival rates (72 vs. 85%, p = 0.021) among the 240 stages II and III colorectal carcinomas by using tissue microarray analysis. Our findings indicate that suppression of SELENBP1 is a frequent and late event in colorectal carcinogenesis, and may contribute to the rapid progression of colorectal carcinoma.
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1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Hoon(김세훈) ORCID logo https://orcid.org/0000-0001-7516-7372
Kim, Tae Il(김태일) ORCID logo https://orcid.org/0000-0003-4807-890X
Kim, Hogeun(김호근)
Song, Si Young(송시영) ORCID logo https://orcid.org/0000-0002-1417-4314
Lee, Kang Young(이강영)
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