Cited 5 times in
An intracellular antibody can suppress tumorigenicity in Hepatitis B virus X-expressing cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신전수 | - |
dc.date.accessioned | 2015-06-10T12:04:26Z | - |
dc.date.available | 2015-06-10T12:04:26Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0340-7004 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/109142 | - |
dc.description.abstract | Although the hepatitis B virus X protein (HBx) is thought to play a causative role in the development of hepatocellular carcinoma, it is not yet known whether interfering with HBx function may affect the cellular transformation of HBx-expressing tumor cells. To address this question, we adopted an intracellular antibody fragment expression approach to block the function of HBx. Expression of a single-chain variable fragment (scFv) specific to HBx (designated as H7scFv) inhibited HBx-dependent cellular transactivation. Furthermore, H7scFv suppressed the growth of HBx-expressing tumor cells in both soft agar and nude mice. The suppressive effect of H7scFv on tumorigenicity appeared not to be mediated by inhibition of HBx-induced growth stimulation since the growth rate of these cells was not affected significantly by H7scFv expression. In conclusion, these data suggest that the HBx-dependent transformed phenotype is reversible and that HBx may be a good molecular target for the treatment of HBV-related tumors. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 569~578 | - |
dc.relation.isPartOf | CANCER IMMUNOLOGY IMMUNOTHERAPY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antibodies, Monoclonal/immunology | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Carcinoma, Hepatocellular/metabolism* | - |
dc.subject.MESH | Carcinoma, Hepatocellular/virology | - |
dc.subject.MESH | Cell Transformation, Neoplastic/metabolism* | - |
dc.subject.MESH | Cytoplasm/immunology | - |
dc.subject.MESH | Fluorescent Antibody Technique | - |
dc.subject.MESH | Immunoglobulin Fragments/immunology* | - |
dc.subject.MESH | Immunoprecipitation | - |
dc.subject.MESH | Liver Neoplasms, Experimental/immunology | - |
dc.subject.MESH | Liver Neoplasms, Experimental/metabolism | - |
dc.subject.MESH | Liver Neoplasms, Experimental/virology | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | NIH 3T3 Cells | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Trans-Activators/immunology | - |
dc.subject.MESH | Trans-Activators/metabolism* | - |
dc.subject.MESH | Transcriptional Activation | - |
dc.title | An intracellular antibody can suppress tumorigenicity in Hepatitis B virus X-expressing cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Young-Hee Jin | - |
dc.contributor.googleauthor | Myung-Hee Kwon | - |
dc.contributor.googleauthor | Kyongmin Kim | - |
dc.contributor.googleauthor | Ho Joon Shin | - |
dc.contributor.googleauthor | Jeon-Soo Shin | - |
dc.contributor.googleauthor | Hyeseong Cho | - |
dc.contributor.googleauthor | Sun Park | - |
dc.identifier.doi | 10.1007/s00262-005-0037-2 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02144 | - |
dc.relation.journalcode | J00445 | - |
dc.identifier.eissn | 1432-0851 | - |
dc.identifier.pmid | 16273352 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs00262-005-0037-2 | - |
dc.subject.keyword | Antibodies | - |
dc.subject.keyword | monoclonal | - |
dc.subject.keyword | viral | - |
dc.subject.keyword | HBxAg | - |
dc.subject.keyword | Cell transformation | - |
dc.subject.keyword | neoplastic | - |
dc.subject.keyword | Hepatocellular carcinoma | - |
dc.contributor.alternativeName | Shin, Jeon Soo | - |
dc.contributor.affiliatedAuthor | Shin, Jeon Soo | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 55 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 569 | - |
dc.citation.endPage | 578 | - |
dc.identifier.bibliographicCitation | CANCER IMMUNOLOGY IMMUNOTHERAPY, Vol.55(5) : 569-578, 2006 | - |
dc.identifier.rimsid | 50617 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.