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Methylation of p16INK4a is a non-rare event in cervical intraepithelial neoplasia

DC Field Value Language
dc.contributor.author김성훈-
dc.contributor.author송기준-
dc.contributor.author조남훈-
dc.contributor.author강숙희-
dc.date.accessioned2015-06-10T12:00:36Z-
dc.date.available2015-06-10T12:00:36Z-
dc.date.issued2006-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/109025-
dc.description.abstractThe cell cycle inhibitor, p16INK4a may be a useful surrogate biomarker of cervical intraepithelial neoplasia (CIN); however, there is currently no consensus of p16INK4a genetic alterations throughout the multiple step process of CIN. Our goal was to identify the methylation frequency of p16INK4a in each step of CIN that is associated with human papillomavirus (HPV) infection, using several different detection methods of p16INK4a methylation to correlate the data. The present study included a total of 43 patients, including 38 with CIN, and 5 normal patients. Three different methods were used to detect hypermethylation of CpG islands, methylation-specific PCR (MSP) amplification of different primer sets of M1, M2, and M3, pyrosequencing of each forward primer region, and immunohistochemistry of p16INK4a. Analysis of MSP showed that 20 of the 38 CIN patients (52.6%) revealed hypermethylation in at least 1 primer set of the p16INK4a promoter. A complete loss of p16INK4a protein expression was observed in 11 cases (28.9%). There was no observed association of methylation of the p16INK4a gene with either CIN grading (P=0.0698) or HPV status (P=0.2811): specifically 42.9% (3/7) was found in CIN 1, 57.1% (8/14) in CIN 2, and 52.9% (9/17) in CIN 3. In concordance with immunohistochemistry results, hypermethylation of the p16INK4a promoter was significantly correlated with a lack of p16 protein expression (P=0.0145). All positive peaks from pyrosequencing matched the MSP results, which ranged from 6.3% to 24.5%. In conclusion, p16INK4a gene silencing during CIN was not determined to be a particularly rare event; however, it does not correlate with either HPV status or CIN grading.-
dc.description.statementOfResponsibilityopen-
dc.format.extent74~82-
dc.relation.isPartOfDIAGNOSTIC PATHOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBase Sequence-
dc.subject.MESHCase-Control Studies-
dc.subject.MESHCervical Intraepithelial Neoplasia/genetics*-
dc.subject.MESHCervical Intraepithelial Neoplasia/metabolism*-
dc.subject.MESHCervical Intraepithelial Neoplasia/pathology-
dc.subject.MESHCervical Intraepithelial Neoplasia/virology-
dc.subject.MESHCpG Islands-
dc.subject.MESHCyclin-Dependent Kinase Inhibitor p16/genetics-
dc.subject.MESHCyclin-Dependent Kinase Inhibitor p16/metabolism-
dc.subject.MESHDNA Methylation*-
dc.subject.MESHDNA Primers/genetics-
dc.subject.MESHDNA, Neoplasm/genetics*-
dc.subject.MESHDNA, Neoplasm/metabolism*-
dc.subject.MESHFemale-
dc.subject.MESHGene Silencing-
dc.subject.MESHGenes, p16*-
dc.subject.MESHHumans-
dc.subject.MESHImmunohistochemistry-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHNeoplasm Proteins/genetics-
dc.subject.MESHNeoplasm Proteins/metabolism-
dc.subject.MESHPapillomaviridae/classification-
dc.subject.MESHPapillomaviridae/isolation & purification-
dc.subject.MESHPolymerase Chain Reaction-
dc.subject.MESHUterine Cervical Neoplasms/genetics*-
dc.subject.MESHUterine Cervical Neoplasms/metabolism*-
dc.subject.MESHUterine Cervical Neoplasms/pathology-
dc.subject.MESHUterine Cervical Neoplasms/virology-
dc.titleMethylation of p16INK4a is a non-rare event in cervical intraepithelial neoplasia-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Obstetrics & Gynecology (산부인과학)-
dc.contributor.googleauthorSuki Kang-
dc.contributor.googleauthorJungsik Kim-
dc.contributor.googleauthorHong Bae Kim-
dc.contributor.googleauthorJung Won Shim-
dc.contributor.googleauthorEunji Nam-
dc.contributor.googleauthorSung Hoon Kim-
dc.contributor.googleauthorHee Jung Ahn-
dc.contributor.googleauthorYoon Pyo Choi-
dc.contributor.googleauthorBoxiao Ding-
dc.contributor.googleauthorKijun Song-
dc.contributor.googleauthorNam Hoon Cho-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02016-
dc.contributor.localIdA03812-
dc.contributor.localIdA00595-
dc.contributor.localIdA00044-
dc.relation.journalcodeJ02909-
dc.identifier.eissn1746-1596-
dc.identifier.pmid16778587-
dc.identifier.urlhttp://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00019606-200606000-00003&LSLINK=80&D=ovft-
dc.subject.keywordp16INK4a-
dc.subject.keywordmethylation-specific PCR (MSP)-
dc.subject.keywordpyrosequencing-
dc.subject.keywordimmunohistochemistry (IHC)-
dc.subject.keywordcervix intraepithelial neoplasia (CIN)-
dc.contributor.alternativeNameKim, Sung Hoon-
dc.contributor.alternativeNameSong, Ki Jun-
dc.contributor.alternativeNameCho, Nam Hoon-
dc.contributor.affiliatedAuthorSong, Ki Jun-
dc.contributor.affiliatedAuthorCho, Nam Hoon-
dc.contributor.affiliatedAuthorKim, Sung Hoon-
dc.contributor.affiliatedAuthorKang, Suki-
dc.rights.accessRightsnot free-
dc.citation.volume15-
dc.citation.number2-
dc.citation.startPage74-
dc.citation.endPage82-
dc.identifier.bibliographicCitationDIAGNOSTIC PATHOLOGY, Vol.15(2) : 74-82, 2006-
dc.identifier.rimsid53721-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biomedical Systems Informatics (의생명시스템정보학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers

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