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성인 중간엽 줄기세포부터 분리된 clone간의 세포증식능력 및 분화능의 다양성 분석

Other Titles
 Clonal Heterogeneity of Human Bone Marrow-Derived Stromal Cells 
Authors
 정호선  ;  김윤희  ;  심인혜  ;  한승환  ;  이진우 
Citation
 Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지), Vol.9(1) : 1-8, 2006 
Journal Title
 Journal of Korean Orthopaedic Research Society (대한정형외과연구학회지) 
ISSN
 1226-8933 
Issue Date
2006
Keywords
Bone marrow-derived stromal cells ; Adipogenesis ; Osteogenesis ; Cell proliferation ; Cell clone
Abstract
Purpose: The isolated human bone marrow-derived stromal cells (BMSCs) were expanded and showed multipotentiality for differentiation to both adipocytes and osteoblasts. We selected the multiple clones and characterized their proliferation potentials, surface markers and differentiation potentials. Materials and Methods: BMSCs were isolated from bone marrow. After forming colonies, colonies were selected and cultured. We performed MTT assay and FACS. Cultured colonies were differentiated into adipocyte and osteoblast. Results: BMSCs were isolated and plated in tissue-culture dishes. The number of clones was about 30. Clones were divided into three groups. Group I was round and small in size, and showed rapid proliferation. Group II showed similar pattern in cell size and proliferation with group I clones during early passage. But, after 4 passages, the clones became flat and larger cells and showed slower proliferation. Finally, group III clones were flat and large cells and replicated slowly in early passage. Group I was fibroblastic in morphology, but Group II changed to flat and larger cells after passage 4. FACS and proliferation assay were performed at passage 5. Group I and Group II cells differed in their expression of the cell-surface epitopes, CD29 and CD105. Group I cells were differentiated into adipocytes better than Group II cells. Conclusion: In this study, these results indicated that cell surface markers were expressed differently for clones having different differentiation properties. These clonal BMSCs may also be used for the identification of lineage-determining factors.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108917
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