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Perillyl alcohol inhibits the expression and function of the androgen receptor in human prostate cancer cells

Authors
 Byung Ha Chung  ;  Hye-young Lee  ;  Jae Seok Lee  ;  Charles Y.F. Young 
Citation
 CANCER LETTERS, Vol.236(2) : 222-228, 2006 
Journal Title
CANCER LETTERS
ISSN
 0304-3835 
Issue Date
2006
MeSH
Androgen Receptor Antagonists* ; Antineoplastic Agents/pharmacology* ; Cell Line, Tumor/drug effects ; Cell Proliferation/drug effects ; Dose-Response Relationship, Drug ; Genes, Reporter ; Humans ; Luciferases/genetics ; Male ; Monoterpenes/pharmacology* ; Nandrolone/analogs & derivatives ; Nandrolone/pharmacology ; Promoter Regions, Genetic/drug effects ; Prostate-Specific Antigen/genetics ; Prostate-Specific Antigen/metabolism ; Prostatic Neoplasms/metabolism* ; Proto-Oncogene Proteins c-jun/biosynthesis ; Proto-Oncogene Proteins c-jun/genetics ; Proto-Oncogene Proteins c-jun/metabolism ; Receptors, Androgen/genetics ; Receptors, Androgen/metabolism ; Tissue Kallikreins/metabolism ; Transcriptional Activation/drug effects ; Transfection
Keywords
Perillyl alcohol ; Androgen receptor ; Prostate carcinoma cell line LNCaP
Abstract
Perillyl alcohol is a hydroxylated monocyclic monoterpene. In animal study, monoterpene has shown to have an anti-tumor effect. The aim of this study is to evaluate whether POH plays an important role in the development and progression of prostate cancer (pCa). We treated LNCaP cells with different concentrations of perillyl alcohol (POH). First of all, we performed cell proliferation assay and prostate-specific antigen (PSA) and human glandular kallikrein (hK2) quantification assays. LNCaP cells were treated with or without POH for Western blot analysis of androgen receptor (AR) and c-Jun. Finally, we performed transient transfection assay by transfecting LNCaP cells—which were treated with or without POH—with pGL-3 luciferase vector containing PSA promoter and AR promoter. We observed inhibition of the expression and function of the AR by POH, through inhibition of androgen-induced cell growth and androgen-stimulated secretion of prostate-specific antigen and hK2, in human pCa cell line LNCaP. In addition, we demonstrated, for the first time, that POH inhibits the transcription activities of the AR gene promoter by over-expression of c-Jun protein. These novel properties of POH strongly suggest that POH could be highly useful for intervention of pCa.
Full Text
http://www.sciencedirect.com/science/article/pii/S0304383505005021
DOI
10.1016/j.canlet.2005.05.023
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Urology (비뇨의학교실) > 1. Journal Papers
Yonsei Authors
Chung, Byung Ha(정병하) ORCID logo https://orcid.org/0000-0001-9817-3660
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108861
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