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Comparative proteomics of pulmonary tumors with neuroendocrine differentiation

Authors
 Nam Hoon Cho  ;  Eun Suk Koh  ;  Dong Wha Lee  ;  Haeryoung Kim  ;  Youn Pyo Choi  ;  Sang Ho Cho  ;  Dong Su Kim 
Citation
 JOURNAL OF PROTEOME RESEARCH, Vol.5(3) : 643-650, 2006 
Journal Title
 JOURNAL OF PROTEOME RESEARCH 
ISSN
 1535-3893 
Issue Date
2006
MeSH
Carcinoma, Large Cell/genetics ; Carcinoma, Large Cell/metabolism ; Carcinoma, Large Cell/pathology ; Carcinoma, Small Cell/genetics ; Carcinoma, Small Cell/metabolism ; Carcinoma, Small Cell/pathology ; Cell Differentiation*/physiology ; Gene Expression Profiling ; Humans ; Lung Neoplasms/genetics ; Lung Neoplasms/metabolism* ; Lung Neoplasms/pathology* ; Neuroendocrine Tumors/genetics ; Neuroendocrine Tumors/metabolism* ; Neuroendocrine Tumors/pathology* ; Proteomics*
Keywords
neuroendocrine pulmonary tumor ; proteomics map tree ; large cell neuroendocrine carcinoma ; small cell carcinoma
Abstract
We aimed to evaluate neuroendocrine pulmonary tumors (NEPT) by a novel method involving map tree construction by comparing all of the protein spots. We performed a proteomics analysis to assess the similarities in protein expression between neuroendocrine pulmonary tumors (NEPT), including typical carcinoids (TC), atypical carcinoids (AC), large cell neuroendocrine carcinomas (LCNEC) and small cell carcinomas (SCLC). Total protein lysates were obtained from seven histologically confirmed frozen NEPT tissues, including 1TC, 2 SCLC, and 4 cases ranging from AC to LCNEC. 2-DE demonstrated that TC was similar to normal lung. AC, LCNEC, and SCLC were similar to each other, forming a group separate from TC, however, SCLC at an early stage showed a similarity to TC. MALDI analysis detected 9 surrogate endpoint biomarkers, including eIF5A1, GST M3, cytokeratin 18 (CK 18), FK506-binding protein p59, p63, MAGE-D2, mitochondrial short-chain enoyl-coenzyme A hydratase 1, tranferrin and poly (rC) binding protein 1. Immunohistochemical staining revealed a gradual decrease in expression rate of p63 and CK 18 with poor differentiation of NEPT. Our results demonstrate that (1) the comparative proteomics of NEPT match the WHO classification except for AC and LCNEC; (2) SCLC show differences in their proteomics according to tumor stage; and (3) CK 18 and p63 may be useful as diagnostically and prognostically available markers.
Full Text
http://pubs.acs.org/doi/abs/10.1021/pr050460x
DOI
10.1021/pr050460x
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Cho, Nam Hoon(조남훈) ORCID logo https://orcid.org/0000-0002-0045-6441
Cho, Sang Ho(조상호)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108750
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