Cited 46 times in
Lysophosphatidic acid receptor 2 and Gi/Src pathway mediate cell motility through cyclooxygenase 2 expression in CAOV-3 ovarian cancer cells
DC Field | Value | Language |
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dc.contributor.author | 이효영 | - |
dc.date.accessioned | 2015-05-19T17:43:27Z | - |
dc.date.available | 2015-05-19T17:43:27Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1226-3613 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/108692 | - |
dc.description.abstract | Lysophosphatidic acid (LPA) is a bioactive phospholipids and involves in various cellular events, including tumor cell migration. In the present study, we investigated LPA receptor and its transactivation to EGFR for cyclooxygenase-2 (COX-2) expression and cell migration in CAOV-3 ovarian cancer cells. LPA induced COX-2 expression in a dose-dependent manner, and pretreatment of the cells with pharmacological inhibitors of Gi (pertussis toxin), Src (PP2), EGF receptor (EGFR) (AG1478), ERK (PD98059) significantly inhibited LPA- induced COX-2 expression. Consistent to these results, transfection of the cells with selective Src siRNA attenuated COX-2 expression by LPA. LPA stimulated CAOV-3 cell migration that was abrogated by pharmacological inhibitors and antibody of EP2. Higher expression of LPA2 mRNA was observed in CAOV-3 cells, and transfection of the cells with a selective LPA2 siRNA significantly inhibited LPA-induced activation of EGFR and ERK, as well as COX-2 expression. Importantly, LPA2 siRNA also blocked LPA-induced ovarian cancer cell migration. Collectively, our results clearly show the significance of LPA2 and Gi/Src pathway for LPA-induced COX-2 expression and cell migration that could be a promising drug target for ovarian cancer cell metastasis | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 607~616 | - |
dc.relation.isPartOf | EXPERIMENTAL AND MOLECULAR MEDICINE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Lysophosphatidic acid receptor 2 and Gi/Src pathway mediate cell motility through cyclooxygenase 2 expression in CAOV-3 ovarian cancer cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | Kang Jin Jeong | - |
dc.contributor.googleauthor | Soon Young Park | - |
dc.contributor.googleauthor | Ji Hye Seo | - |
dc.contributor.googleauthor | Kyung Bok Lee | - |
dc.contributor.googleauthor | Wahn Soo Choi | - |
dc.contributor.googleauthor | Jeung Whan Han | - |
dc.contributor.googleauthor | Jae Ku Kang | - |
dc.contributor.googleauthor | Chang Gyo Park | - |
dc.contributor.googleauthor | Yong Kee Kim | - |
dc.contributor.googleauthor | Hoi Young Lee | - |
dc.identifier.doi | 10.3858/emm.2008.40.6.607 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03341 | - |
dc.relation.journalcode | J00860 | - |
dc.identifier.eissn | 2092-6413 | - |
dc.identifier.pmid | cell movement; cyclooxygenase-2; lysophosphatidic acid; ovarian neoplasms; proto-oncogene proteins pp60 (c-src); receptors; lysophosphatidic acid | - |
dc.subject.keyword | cell movement | - |
dc.subject.keyword | cyclooxygenase-2 | - |
dc.subject.keyword | lysophosphatidic acid | - |
dc.subject.keyword | ovarian neoplasms | - |
dc.subject.keyword | proto-oncogene proteins pp60 (c-src) | - |
dc.subject.keyword | receptors | - |
dc.subject.keyword | lysophosphatidic acid | - |
dc.contributor.alternativeName | Lee, Hoi Young | - |
dc.contributor.affiliatedAuthor | Lee, Hoi Young | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 40 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 607 | - |
dc.citation.endPage | 616 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL AND MOLECULAR MEDICINE, Vol.40(6) : 607-616, 2008 | - |
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