Cited 6 times in
Identification of novel metronidazole-inducible genes in Mycobacterium smegmatis using a customized amplification library.
DC Field | Value | Language |
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dc.contributor.author | 신성재 | - |
dc.date.accessioned | 2015-05-19T17:41:56Z | - |
dc.date.available | 2015-05-19T17:41:56Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0378-1097 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/108645 | - |
dc.description.abstract | The incidence of antibiotic resistance in pathogenic bacteria is rising. Bacterial resistance may be a natural defense of organisms, or it may result from spontaneous mutations or the acquisition of exogenous resistance genes. We grew spontaneous metronidazole-resistant Mycobacterium smegmatis mutants on solid medium cultures and employed differential expression using a customized amplification library to analyze the global gene profiles of metronidazole-resistant mutants under hypoxic conditions. In total, 66 genes involved in metronidazole resistance were identified and functionally characterized using the gene role category of M. smegmatis. Overall, genes associated with cell wall synthesis, such as methyltransferase and glycosyltransferase, and genes encoding drug transporters were highly expressed. The genes may be involved in the natural drug resistance of mycobacteria by increasing mycobacterial cell wall permeability and the efflux pumps of active drugs. In addition, the genes may play a role in dormancy. The genes identified in this study may lead to a better understanding of the mechanisms of metronidazole resistance during dormancy | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 282~289 | - |
dc.relation.isPartOf | FEMS MICROBIOLOGY LETTERS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Drug Resistance, Bacterial/genetics* | - |
dc.subject.MESH | Gene Expression Regulation, Bacterial | - |
dc.subject.MESH | Gene Library | - |
dc.subject.MESH | Genes, Bacterial/drug effects* | - |
dc.subject.MESH | Genes, Bacterial/physiology | - |
dc.subject.MESH | Metronidazole/pharmacology* | - |
dc.subject.MESH | Mycobacterium smegmatis/drug effects | - |
dc.subject.MESH | Mycobacterium smegmatis/genetics* | - |
dc.subject.MESH | Mycobacterium smegmatis/metabolism | - |
dc.title | Identification of novel metronidazole-inducible genes in Mycobacterium smegmatis using a customized amplification library. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Su-Young Kim | - |
dc.contributor.googleauthor | Sung Jae Shin | - |
dc.contributor.googleauthor | Chang-Hwa Song | - |
dc.contributor.googleauthor | Eun-Kyeong Jo | - |
dc.contributor.googleauthor | Hwa-Jung Kim | - |
dc.contributor.googleauthor | Jeong-Kyu Park | - |
dc.identifier.doi | 10.1111/j.1574-6968.2008.01143.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02114 | - |
dc.relation.journalcode | J00892 | - |
dc.identifier.eissn | 1574-6968 | - |
dc.identifier.pmid | 18373646 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2008.01143.x/abstract | - |
dc.subject.keyword | Mycobacterium smegmatis | - |
dc.subject.keyword | DECAL | - |
dc.subject.keyword | metronidazole resistant | - |
dc.contributor.alternativeName | Shin, Sung Jae | - |
dc.contributor.affiliatedAuthor | Shin, Sung Jae | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 282 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 282 | - |
dc.citation.endPage | 289 | - |
dc.identifier.bibliographicCitation | FEMS MICROBIOLOGY LETTERS, Vol.282(2) : 282-289, 2008 | - |
dc.identifier.rimsid | 37150 | - |
dc.type.rims | ART | - |
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