한랭이 피부장벽에 미치는 영향

Abstract

Background: Cold is considered an important environmental factor on the skin's barrier function. It is also known that cold can disrupt the barrier's permeability function. Historically irritant contact dermatitis develops frequently amongst fish pr℃essing workers. This means that cold may disrupt the skin barrier function. However, there are only a few studies of the effect of cold on the skin barrier. Objective: The aim of our study was to investigate the effects of exposure to the cold on the skin''s barrier permeability, calcium gradient and cornified enveloped proteins. Methods: Hairless mice were classified into four groups as follows; tape-stripping and exposure to cold (4℃) for 5 hours group (Group 1), exposure to cold (4℃) for 5 hours after tape-stripping and occlusion with Gore-Tex group (Group 2), exposure to cold (4℃) for 5 hours after tape-stripping and applying with petrolatum group (Group 3), and tape-stripping and exposure at room temperature (25℃) for 5 hours group (Group 4). Next, rate of barrier recovery was measured, and skin of cold exposure site was taken for immunohistochemistry of cornified enveloped protein, calcium inon capture chemistry and in situ hybridization. Results: Rate of barrier recovery of Group 1 was measured at -56.33%, the most delayed rate of all groups. Rate of barrier recovery of other groups were measured as follows; Group 2 (20%), Group 3 (55%) and Group 4 (41.78%). In calcium-ion capture cytochemistry, Group 1 appeared to absent calcium deposit in the entire epidermis. Group 3 appeared to the highest density of calcium in entire epidermis. In immunohistochemical staining of cornified enveloped proteins including involucrin, loricrin and filaggrin, Group 1 resulted in the lowest expression of cornified enveloped proteins. Of all groups, Group 3 appeared to have the highest expressionof cornified enveloped proteins. In situ hybridization of loricrin and filaggrin, expression were similar with results of immunohistochemical staining. Conclusion: Our results provide that cold impairs the skin's barrier permeability by having a negative effect on recovery of calcium gradient and differentiation of cornifed enveloped proteins. But occlusion with vapor permeable membranes such as Gore-Tex or petrolatum could improve conditions of skin barrier impaired by cold by restoring the recovery rate of calcium gradient and differentiation of cornified enveloped protein