Cited 3 times in
Growth factor-free cultured rat bone marrow derived mesenchymal stem cells towards hepatic progenitor cell differentiation
DC Field | Value | Language |
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dc.contributor.author | 김재형 | - |
dc.contributor.author | 서활 | - |
dc.contributor.author | 신상현 | - |
dc.contributor.author | 조현희 | - |
dc.date.accessioned | 2015-05-19T17:34:42Z | - |
dc.date.available | 2015-05-19T17:34:42Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 1226-8372 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/108412 | - |
dc.description.abstract | Induction of mesenchymal stem cells (MSCs) differentiation by growth factors is not likely to be acceptable for clinical application. In this study, MSCs and hepatocytes isolated from male Sprague-Dawley rats were indirectly co-cultured by sharing media, without any growth factor, for 14 days. Differentiation was investigated by detection of hepatic markers, albumin, α-fetoprotein (AFP), cytokeratin-18 (CK18), and bile ductal epithelial cell marker cytokeratin-19 (CK19) on reverse transcription polymerase chain reaction (RT-PCR), immunofluorescence staining, and Western blot. Periodic acid-schiff (PAS) staining, low density lipoproteins (LDL) uptake analysis, and urea assay were performed to detect metabolic activities of differentiated cells. MSCs in co-culture group showed positive expression for albumin, AFP, and CK19, but little for CK18. Data indicates that co-cultured MSCs were differentiated into oval cell stage, the hepatic progenitor, but not directly toward complete hepatocytes. Our finding suggests that indirect co-culture system has potential to generate regenerative hepatocytes by hepatic progenitor cells differentiated from MSCs | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 659~665 | - |
dc.relation.isPartOf | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Growth factor-free cultured rat bone marrow derived mesenchymal stem cells towards hepatic progenitor cell differentiation | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Medical Engineering (의학공학) | - |
dc.contributor.googleauthor | Tian Zhu Li | - |
dc.contributor.googleauthor | Sang-Hyun Shin | - |
dc.contributor.googleauthor | Hyun Hee Cho | - |
dc.contributor.googleauthor | Jae Hyung Kim | - |
dc.contributor.googleauthor | Hwal Suh | - |
dc.identifier.doi | 10.1007/s12257-008-0080-x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01924 | - |
dc.contributor.localId | A02106 | - |
dc.contributor.localId | A03934 | - |
dc.contributor.localId | A00873 | - |
dc.relation.journalcode | J00334 | - |
dc.identifier.eissn | 1976-3816 | - |
dc.identifier.pmid | co-culture; growth factor-free; hepatic progenitor cell differentiation; liver regeneration; mesenchymal stem cells; oval cells | - |
dc.identifier.url | http://link.springer.com/article/10.1007/s12257-008-0080-x# | - |
dc.subject.keyword | co-culture | - |
dc.subject.keyword | growth factor-free | - |
dc.subject.keyword | hepatic progenitor cell differentiation | - |
dc.subject.keyword | liver regeneration | - |
dc.subject.keyword | mesenchymal stem cells | - |
dc.subject.keyword | oval cells | - |
dc.contributor.alternativeName | Kim, Jae Hyung | - |
dc.contributor.alternativeName | Suh, Hwal | - |
dc.contributor.alternativeName | Shin, Sang Hyun | - |
dc.contributor.alternativeName | Cho, Hyun Hee | - |
dc.contributor.affiliatedAuthor | Suh, Hwal | - |
dc.contributor.affiliatedAuthor | Shin, Sang Hyun | - |
dc.contributor.affiliatedAuthor | Cho, Hyun Hee | - |
dc.contributor.affiliatedAuthor | Kim, Jae Hyung | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 13 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 659 | - |
dc.citation.endPage | 665 | - |
dc.identifier.bibliographicCitation | BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, Vol.13(6) : 659-665, 2008 | - |
dc.identifier.rimsid | 35554 | - |
dc.type.rims | ART | - |
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