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염색체성 AmpC β-lactamase 생성 Enterobacteriaceae 균종에서의 CTX-M형 extended-spectrum β-lactamase 검출과 분자유전학적 특성 규명

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dc.contributor.author김창기-
dc.contributor.author염종화-
dc.contributor.author용동은-
dc.contributor.author이경원-
dc.contributor.author정석훈-
dc.contributor.author정윤섭-
dc.date.accessioned2015-05-19T17:33:02Z-
dc.date.available2015-05-19T17:33:02Z-
dc.date.issued2008-
dc.identifier.issn1229-0025-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/108357-
dc.description.abstractBackground: Clinical isolates of AmpC β-lactamase producing Enterobacteriaceae were evaluated to determine the prevalence of CTX-M extended-spectrum β-lactamases (ESBLs) and their genetic environments. Methods: A total of 250 non-duplicate isolates of Eneterobacter aerogenes, E. cloacae, Citrobacter freundii, Serratia marcescens and Morganella morganii were collected at a Korean hospital. ESBL production was determined by double disk synergy test. For ESBL producers, bla genes were sequenced and blaCTX-M environment was characterized by PCR mapping and sequencing. Results: Among the 250 isolates 29 (11.6%) produced ESBL, and 14 of the 29 isolates produced CTX-M ESBLs, including CTX-M-9 by 8 isolates, CTX-M-3 by 4 isolates, CTX-M-12 by 1 isolate, and CTX-M-14 by 1 isolate. ISEcp1 was present upstream of blaCTX-M-3, 12, and 14. Three of the four CTXM-3 producers had the same genetic environment (pemK-ISEcp1-blaCTX-M-3-orf477-mucA). An IS903-like element was found downstream of blaCTX-M-14. ISCR1 was identified upstream of blaCTX-M-9 and ISCR1 and blaCTX-M-9 were located on sul1-type class 1 integron. The variable region between the 5’-CS and the first 3’-CS contained dfrA16 and aadA2. Its structure was similar to that of In60, but our isolates did not have IS3000 or second 3’-CS. Conclusion: CXT-M type ESBL was prevalent in AmpC β-lactamase-producing Enterobacteriaceae, particularly E. cloacae. blaCTX-M genes were associated with ISEcp1 or ISCR1. This is the first report on the genetic environment of blaCTX-M in Korean isolates.-
dc.description.statementOfResponsibilityopen-
dc.format.extent90~97-
dc.relation.isPartOfKorean Journal of Clinical Microbiology (대한임상미생물학회지)-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.title염색체성 AmpC β-lactamase 생성 Enterobacteriaceae 균종에서의 CTX-M형 extended-spectrum β-lactamase 검출과 분자유전학적 특성 규명-
dc.title.alternativeDetection of CTX-M-Type Extended-Spectrum β-Lactamase in Clinical Isolates of Chromosomal AmpC β-Lactamase-Producing Enterobacteriaceae from Korea and Their Molecular Characteristics-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthor김창기-
dc.contributor.googleauthor염종화-
dc.contributor.googleauthor용동은-
dc.contributor.googleauthor정석훈-
dc.contributor.googleauthor이경원-
dc.contributor.googleauthor정윤섭-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01038-
dc.contributor.localIdA02352-
dc.contributor.localIdA02423-
dc.contributor.localIdA02649-
dc.contributor.localIdA03619-
dc.contributor.localIdA03679-
dc.relation.journalcodeJ01984-
dc.identifier.pmidExtended-spectrum β-lactamase ; CTXM ; Genetic characteristics-
dc.subject.keywordExtended-spectrum β-lactamase-
dc.subject.keywordCTXM-
dc.subject.keywordGenetic characteristics-
dc.contributor.alternativeNameKim, Chang Ki-
dc.contributor.alternativeNameYum, Jong Hwa-
dc.contributor.alternativeNameYong, Dong Eun-
dc.contributor.alternativeNameLee, Kyung Won-
dc.contributor.alternativeNameJeong, Seok Hoon-
dc.contributor.alternativeNameChong, Yun Sop-
dc.contributor.affiliatedAuthorKim, Chang Ki-
dc.contributor.affiliatedAuthorYum, Jong Hwa-
dc.contributor.affiliatedAuthorYong, Dong Eun-
dc.contributor.affiliatedAuthorLee, Kyung Won-
dc.contributor.affiliatedAuthorJeong, Seok Hoon-
dc.contributor.affiliatedAuthorChong, Yun Sop-
dc.rights.accessRightsfree-
dc.citation.volume11-
dc.citation.number2-
dc.citation.startPage90-
dc.citation.endPage97-
dc.identifier.bibliographicCitationKorean Journal of Clinical Microbiology (대한임상미생물학회지), Vol.11(2) : 90-97, 2008-
dc.identifier.rimsid35513-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers

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