천식과 아토피피부염 환자에서 D. farinae 특이 IgE 결합 양상의 차이

Abstract

Background: Dermatophagoides farinae (D. farinae) has various isoallergens. However, allergenic differences between bronchial asthmatics (BA) and atopic dermatitis (AD) patients have not yet been fully evaluated. Objective: To investigate the differences in D. farinae specific IgE between BA and AD patients sensitized to D. farinae. Method: Five BA and five AD patients sensitized to D. farinae were measured in this study. D. farinae-specific IgE was evaluated by 1-dimensional (1-D) and 2-dimensional (2-D) SDS-PAGE, and IgE immunoblotting. N-terminal sequencing was performed for the identification of the allergens. Result: Two-D IgE immunoblot revealed the 7 allergens with 14 kDa at PI 5.5 to 6.5. These allergens were found in 4, BA and 2 AD patients. Another allergen with 25 kDa at pI 7.8 was found only in 4 BA patients. This allergen was regarded as triosephosphate isomerase by N-terminal sequencing. Conclusion: Seven allergens with 14 kDa at pI 5.5 to 6.5 and 1 allergen with 25 kDa at pI 7.8 were identified more frequently in BA than in AD patients. The 25 kDa allergen was regarded as triosephosphate isomerase, and it might be associated with the development of asthma.