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Biopositive Effects of Low-Dose UVB on Epidermis: Coordinate Upregulation of Antimicrobial Peptides and Permeability Barrier Reinforcement

Authors
 Seung P. Hong  ;  Min J. Kim  ;  Min-young Jung  ;  Hyerin Jeon  ;  Jawoong Goo  ;  Sung K. Ahn  ;  Seung H. Lee  ;  Peter M. Elias  ;  Eung H. Choi 
Citation
 JOURNAL OF INVESTIGATIVE DERMATOLOGY, Vol.128(12) : 2880-2887, 2008 
Journal Title
 JOURNAL OF INVESTIGATIVE DERMATOLOGY 
ISSN
 0022-202X 
Issue Date
2008
MeSH
Animals ; Antimicrobial Cationic Peptides/pharmacology* ; Cathelicidins ; Cell Differentiation ; Cholecalciferol/metabolism ; Epidermis/immunology* ; Epidermis/radiation effects* ; Female ; Immunohistochemistry ; Ketoconazole/pharmacology ; Lipids/chemistry ; Mice ; Mice, Hairless ; Models, Biological ; Permeability ; Receptors, Calcitriol/metabolism ; Ultraviolet Rays
Keywords
Animals ; Antimicrobial Cationic Peptides/pharmacology* ; Cathelicidins ; Cell Differentiation ; Cholecalciferol/metabolism ; Epidermis/immunology* ; Epidermis/radiation effects* ; Female ; Immunohistochemistry ; Ketoconazole/pharmacology ; Lipids/chemistry ; Mice ; Mice, Hairless ; Models, Biological ; Permeability ; Receptors, Calcitriol/metabolism ; Ultraviolet Rays
Abstract
Whereas high-dose ultraviolet B (UVB) is detrimental to the epidermal permeability barrier, suberythemal doses of UVB are used to treat atopic dermatitis (AD), which is characterized by defective permeability barrier and antimicrobial function. As epidermal permeability barrier and antimicrobial peptide (AMP) expression are coregulated and interdependent functions, we hypothesized that suberythemal doses of UVB exposure could regulate AMP expression in parallel with permeability barrier function. Hairless mice were exposed to 40 mJ cm(-2) UVB (about 1/2 minimal erythema dose) daily for 1 or 3 days. Twenty-four hours after the last exposure, epidermal barrier function was assessed and skin specimens were taken for western blotting, immunohistochemistry, and quantitative reverse transcription-PCR for mouse beta-defensin (mBD)-2, mBD3 and cathelin-related antimicrobial peptide (CRAMP). mRNA levels of the vitamin D receptor (VDR), 1alpha-hydroxylase and key epidermal lipid synthetic enzymes were also quantified. After 3 days of UVB exposure, acceleration of barrier recovery and augmentation in expression of epidermal differentiation markers (for example, involucrin and filaggrin) occurred in parallel with increased mBD2, mBD3, and CRAMP expression at both the mRNA and protein level. VDR, 1alpha-hydroxylase, and the major epidermal lipid synthetic enzymes were also upregulated. When an inhibitor of 1alpha, 25 dihydroxyvitamin D(3) formation, ketoconazole, was applied immediately after UVB exposure, the cutaneous vitamin D system was inhibited, which in turn blocked epidermal lipid synthesis, AMP expression, and permeability barrier homeostasis, suggesting that the beneficial effect of low-dose UVB depends, at least in part, on activation of the cutaneous vitamin D system. Our results provide new insights into the mechanisms whereby low-dose UVB comprises effective therapy for AD.
Files in This Item:
T200804342.pdf Download
DOI
10.1038/jid.2008.169
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Seung Hun(이승헌)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/108257
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