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Interaction of SOCS3 with NonO attenuates IL-1beta-dependent MUC8 gene expression.

DC FieldValueLanguage
dc.contributor.author김규보-
dc.contributor.author송경섭-
dc.contributor.author윤주헌-
dc.date.accessioned2015-05-19T17:14:06Z-
dc.date.available2015-05-19T17:14:06Z-
dc.date.issued2008-
dc.identifier.issn0006-291X-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/107759-
dc.description.abstractThe intracellular negatively regulatory mechanism which affects IL-1beta-induced MUC8 gene expression remains unclear. We found that SOCS3 overexpression suppressed IL-1beta-induced MUC8 gene expression in NCI-H292 cells, whereas silencing of SOCS3 restored IL-1beta-induced MUC8 gene expression. Sequentially activated ERK1/2, RSK1, and CREB by IL-1beta were not affected by SOCS3, indicating that SOCS3 has an independent mechanism of action. Using immunoprecipitaion and nano LC mass analysis, we found that SOCS3 bound NonO (non-POU-domain containing, octamer-binding domain protein) in the absence of IL-1beta, whereas IL-1beta treatment dissociated the direct binding of SOCS3 and NonO. A dominant-negative SOCS3 mutant (Y204F/Y221F) did not bind to NonO. Interestingly, SOCS3 overexpression dramatically suppressed MUC8 gene expression in cells transfected with wild-type or siRNA of NonO. Moreover, silencing of SOCS3 dramatically increased NonO-mediated MUC8 gene expression caused by IL-1beta compared to NonO overexpression alone, suggesting that SOCS3 acts as a suppressor by regulating the action of NonO.-
dc.description.statementOfResponsibilityopen-
dc.format.extent946~951-
dc.relation.isPartOfBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHCell Line-
dc.subject.MESHGene Expression-
dc.subject.MESHHumans-
dc.subject.MESHInterleukin-1beta/genetics-
dc.subject.MESHInterleukin-1beta/metabolism*-
dc.subject.MESHMucins/genetics*-
dc.subject.MESHNuclear Matrix-Associated Proteins/genetics-
dc.subject.MESHNuclear Matrix-Associated Proteins/metabolism*-
dc.subject.MESHOctamer Transcription Factors/genetics-
dc.subject.MESHOctamer Transcription Factors/metabolism*-
dc.subject.MESHRNA-Binding Proteins/genetics-
dc.subject.MESHRNA-Binding Proteins/metabolism*-
dc.subject.MESHSuppressor of Cytokine Signaling 3 Protein-
dc.subject.MESHSuppressor of Cytokine Signaling Proteins/antagonists & inhibitors-
dc.subject.MESHSuppressor of Cytokine Signaling Proteins/genetics-
dc.subject.MESHSuppressor of Cytokine Signaling Proteins/metabolism*-
dc.subject.MESHTranscriptional Activation*-
dc.subject.MESHUbiquitination-
dc.titleInteraction of SOCS3 with NonO attenuates IL-1beta-dependent MUC8 gene expression.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Otorhinolaryngology (이비인후과학)-
dc.contributor.googleauthorKyoung Seob Song-
dc.contributor.googleauthorKyubo Kim-
dc.contributor.googleauthorKwang Chul Chung-
dc.contributor.googleauthorJae Hong Seol-
dc.contributor.googleauthorJoo-Heon Yoon-
dc.identifier.doi10.1016/j.bbrc.2008.10.084-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00323-
dc.contributor.localIdA02604-
dc.contributor.localIdA02010-
dc.relation.journalcodeJ00281-
dc.identifier.eissn1090-2104-
dc.identifier.pmid18952062-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0006291X08020755-
dc.subject.keywordIL-1β-
dc.subject.keywordInflammation-
dc.subject.keywordMUC8-
dc.subject.keywordSOCS3-
dc.subject.keywordNonO-
dc.contributor.alternativeNameKim, Kyu Bo-
dc.contributor.alternativeNameSong, Kyoung Seob-
dc.contributor.alternativeNameYoon, Joo Heon-
dc.contributor.affiliatedAuthorKim, Kyu Bo-
dc.contributor.affiliatedAuthorYoon, Joo Heon-
dc.contributor.affiliatedAuthorSong, Kyoung Seob-
dc.rights.accessRightsnot free-
dc.citation.volume377-
dc.citation.number3-
dc.citation.startPage946-
dc.citation.endPage951-
dc.identifier.bibliographicCitationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.377(3) : 946-951, 2008-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Airway Hucus Institute (기도점액연구소) > 1. Journal Papers

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