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Genomic and proteomic characterization of YDOV-157, a newly established human epithelial ovarian cancer cell line.

 HanByoul Cho  ;  Eun Suk Kang  ;  Soon Won Hong  ;  Youn Jin Oh  ;  Sun Mi Choi  ;  Sang Wun Kim  ;  Sung Hoon Kim  ;  Young Tae Kim  ;  Kyoung Sun Lee  ;  Yang Kyu Choi  ;  Jae-Hoon Kim 
 MOLECULAR AND CELLULAR BIOCHEMISTRY, Vol.319(1-2) : 189-201, 2008 
Journal Title
Issue Date
Adenocarcinoma/metabolism* ; Adenocarcinoma/pathology ; Cell Line, Tumor/metabolism* ; Cell Line, Tumor/pathology ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic* ; Humans ; Models, Biological* ; Neoplasm Proteins/biosynthesis* ; Oligonucleotide Array Sequence Analysis ; Ovarian Neoplasms/metabolism* ; Ovarian Neoplasms/pathology ; Proteomics/methods
Ovarian cancer ; Cell line ; Characterization ; Microarray ; 2D electrophoresis
The existence of several model systems with which to investigate a particular disease is advantageous for researchers. This is especially true for ovarian cancer, which, due to its complex and heterogeneous nature, inherently requires a large number of model systems. Here, we report a new ovarian serous adenocarcinoma cell line, designated YDOV-157, and characterized via post genomics and post proteomics. In this study, primary culture of tumor cells from ascites was performed and the cells were immortalized up to at least 60 passages in vitro. We studied the morphologies, cell proliferation, BRCA1/2 mutations, tumorigenesis capacity, and chemosensitivity of YDOV-157. Using a cDNA microarray, differentially expressed genes were identified and some of them were validated. Using proteomic analysis, we identified proteins that were differentially expressed in YDOV-157. The newly derived cell line, designated YDOV-157, grew as a monolayer and the doubling time was 102 h. When transplanted into nude mice, it initiated the formation of tumor masses with microscopic findings identical to those of the primary tumor. Chemosensitivity test showed that paclitaxel induced the highest chemosensitivity index. In microarray analysis, 2,520 probes were differently expressed, compared to human ovarian surface epithelial cells (HOSEs). In SYBR Green real-time PCR, the expression of E2F2 (P = 0.040) and CRABP2 genes (P = 0.030) was significantly higher in the ovarian cancer cell lines than in HOSEs. Furthermore, proteomic analysis showed that expression of 28 spots was significantly altered between YDOV-157 and HOSE. In conclusion, the newly derived YDOV-157 cell line may be an important research resource for studying cancer cell biology and should also be very useful for developing new strategies that inhibit cancer cell growth and progression
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1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sang Wun(김상운) ORCID logo https://orcid.org/0000-0002-8342-8701
Kim, Sung Hoon(김성훈) ORCID logo https://orcid.org/0000-0002-1645-7473
Kim, Young Tae(김영태) ORCID logo https://orcid.org/0000-0002-7347-1052
Kim, Jae Hoon(김재훈) ORCID logo https://orcid.org/0000-0001-6599-7065
Cho, Hanbyoul(조한별) ORCID logo https://orcid.org/0000-0002-6177-1648
Hong, Soon Won(홍순원) ORCID logo https://orcid.org/0000-0002-0324-2414
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