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Epigallocatechin-3-gallate inhibits interleukin-1beta-induced MUC5AC gene expression and MUC5AC secretion in normal human nasal epithelial cells.

 Hyun Jik Kim  ;  Sang Ho Park  ;  Sung-Yoon Park  ;  Uk Yeol Moon  ;  Byung Don Lee  ;  Sung Hyun Yoon  ;  Jeung-Gweon Lee  ;  Seung Joon Baek  ;  Joo-Heon Yoon 
 Journal of Nutritional Biochemistry, Vol.19(8) : 536-544, 2008 
Journal Title
 Journal of Nutritional Biochemistry 
Issue Date
Catechin/analogs & derivatives* ; Catechin/pharmacology ; Dose-Response Relationship, Drug ; Enzyme Activation/drug effects ; Gene Expression/drug effects* ; Humans ; Interleukin-1beta/antagonists & inhibitors* ; Interleukin-1beta/pharmacology ; Lung Neoplasms ; Mitogen-Activated Protein Kinase 1/metabolism ; Mitogen-Activated Protein Kinase 3/metabolism ; Mucin 5AC ; Mucins/genetics* ; Mucins/metabolism* ; Nasal Mucosa/drug effects* ; Nasal Mucosa/metabolism ; Promoter Regions, Genetic/drug effects ; Signal Transduction/drug effects ; Tumor Cells, Cultured
It has been reported that the proinflammatory cytokine interleukin-1beta (IL-1beta) induces mucus hypersecretion in normal human nasal epithelial (NHNE) cells and that the MAP kinase pathway may be an important signal pathway in IL-1beta-induced MUC5AC gene expression. Green tea (Camellia sinensis) polyphenols are potent anti-inflammatory agents and have been shown to inhibit inflammation in tumor cell lines and cultured respiratory epithelial cells. In this study, we examined the effect of (-)-epigallocatechin-3-gallate (EGCG), a green tea polyphenol, on IL-1beta-induced MUC5AC gene expression and secretion in NHNE cells. After cells had been treated with IL-1beta (10 ng/ml) and pretreated with EGCG (10, 50 and 100 microM), mRNA expression of MUC5AC was determined by real-time polymerase chain reaction. The suppression of each signal pathway protein was determined by Western blot analysis after treatment with IL-1beta and EGCG, respectively. IL-1beta increased MUC5AC gene expression and MUC5AC secretion. EGCG markedly suppressed IL-1beta-induced MUC5AC gene expression and MUC5AC secretion via suppression of the phosphorylation of ERK MAP kinase, MSK1, and transcription factor, cAMP response element-binding protein. IL-1beta increased the number of cells staining positive with MUC5AC antibodies, and EGCG treatment decreased this number. Our data suggest that EGCG may be an effective inhibitor of IL-1beta-induced mucus hypersecretion.
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1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Joo Heon(윤주헌)
Lee, Jeung Gweon(이정권)
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