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The role of high-mobility group box-1 in renal ischemia and reperfusion injury and the effect of ethyl pyruvate

Authors
 K.-Y. Chung  ;  J.-J. Park  ;  Y.S. Kim 
Citation
 TRANSPLANTATION PROCEEDINGS, Vol.40(7) : 2136-2138, 2008 
Journal Title
TRANSPLANTATION PROCEEDINGS
ISSN
 0041-1345 
Issue Date
2008
MeSH
Animals ; Creatinine/blood ; HMGB1 Protein/metabolism ; HMGB1 Protein/physiology* ; Ischemia/physiopathology* ; Ischemia/prevention & control* ; Pyruvates/therapeutic use* ; Rats ; Rats, Sprague-Dawley ; Renal Circulation/drug effects ; Renal Circulation/physiology* ; Reperfusion Injury/physiopathology* ; Tumor Necrosis Factor-alpha/drug effects ; Tumor Necrosis Factor-alpha/physiology
Keywords
Animals ; Creatinine/blood ; HMGB1 Protein/metabolism ; HMGB1 Protein/physiology* ; Ischemia/physiopathology* ; Ischemia/prevention & control* ; Pyruvates/therapeutic use* ; Rats ; Rats, Sprague-Dawley ; Renal Circulation/drug effects ; Renal Circulation/physiology* ; Reperfusion Injury/physiopathology* ; Tumor Necrosis Factor-alpha/drug effects ; Tumor Necrosis Factor-alpha/physiology
Abstract
PURPOSE:

High mobility group box-1(HMGB1) was identified as a DNA-binding protein that functions as a cofactor for proper transcriptional regulation in somatic cells. Extracellular HMGB1 acts as a potent proinflammatory cytokine that contributes to the pathogenesis of diverse inflammatory and infectious disorders. Ethyl pyruvate (EP), a stable aliphatic ester derived from pyruvic acid, was first described as a pharmacological inhibitor of HMGB1 secretion. We designed this study to identify changes in HMGB1 expression in rat kidney tissues after ischemia reperfusion injury and effects of EP on the expression of HMGB1.

MATERIALS AND METHODS:

Sprague-Dawley rats (200-300 g) were subjected to 40 minutes of renal warm ischemia. The animals were divided into 3 groups: sham group without warm ischemia, EP group (EP given before ischemia), and ischemic control group. Kidneys were harvested and serum creatinine and TNF-alpha measured at 6 hours, 1 day, 3 days, and 5 days after reperfusion. We performed immunohistochemical staining of HMGB1.

RESULTS:

Serum creatinine and TNF-alpha level were elevated in the ischemic control group and the EP injection group. In the EP injection group, serum creatinine and TNF-alpha levels were lower than the ischemic control group. In the 40-minute ischemia-reperfusion model, HMGB1 expression increased at 6 hours after reperfusion and decreased gradually at 1, 3, and 5 days after reperfusion. HMGB1 expression was more distinct at the outer medullary area. intraperitoneal EP injection had no effect on HMGB1 expression.

CONCLUSION:

From these results, we deduced that the preventive effect of EP on rat kidney ischemia-reperfusion injury was not due to the decreased expression of HMGB1 but the prevention of HMGB1 release
Full Text
http://www.sciencedirect.com/science/article/pii/S0041134508007872
DOI
10.1016/j.transproceed.2008.06.040
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Yu Seun(김유선) ORCID logo https://orcid.org/0000-0002-5105-1567
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/107588
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