4 218

Cited 17 times in

Elevated anti-alpha-enolase antibody levels in Kawasaki disease

Authors
 J-K Chun  ;  TJ Lee  ;  KM Choi  ;  KH Lee  ;  DS Kim 
Citation
 SCANDINAVIAN JOURNAL OF RHEUMATOLOGY, Vol.37(1) : 48-52, 2008 
Journal Title
 SCANDINAVIAN JOURNAL OF RHEUMATOLOGY 
ISSN
 0300-9742 
Issue Date
2008
MeSH
Acute Disease ; Autoantibodies/blood* ; Enzyme-Linked Immunosorbent Assay ; Fever/blood ; Fever/immunology ; Humans ; Immunoglobulin M/blood ; Inflammation/blood ; Inflammation/immunology ; Mucocutaneous Lymph Node Syndrome/blood ; Mucocutaneous Lymph Node Syndrome/immunology* ; Mucocutaneous Lymph Node Syndrome/physiopathology ; Phosphopyruvate Hydratase/immunology*
Keywords
Acute Disease ; Autoantibodies/blood* ; Enzyme-Linked Immunosorbent Assay ; Fever/blood ; Fever/immunology ; Humans ; Immunoglobulin M/blood ; Inflammation/blood ; Inflammation/immunology ; Mucocutaneous Lymph Node Syndrome/blood ; Mucocutaneous Lymph Node Syndrome/immunology* ; Mucocutaneous Lymph Node Syndrome/physiopathology ; Phosphopyruvate Hydratase/immunology*
Abstract
Objective: By functioning as a heat‐shock protein (HSP), α‐enolase has an important role in the pathophysiology of multivariant vasculitis. Kawasaki disease (KD) is a type of vasculitis occurring primarily in children. The role of α‐enolase in KD was assessed by measuring anti‐α‐enolase antibody (Ab) titres in patients with KD and the usefulness of anti‐α‐enolase Ab as a diagnostic tool in atypical KD patients was evaluated. Methods: Anti‐α‐enolase Ab titres were measured by using an enzyme‐linked immunosorbent assay (ELISA) in seven normal control patients, nine febrile control patients and 14 KD patients (10 typical KD, four atypical KD). A standard deviation (SD) of 3 above the mean of the normal control group was considered to be positive reactivity. Western blotting using recombinant human α‐enolase was performed in four KD patients and three normal controls. Results: With the positive reactivity limited to +3 SD over the mean (>0.6), 10 out of 14 patients (71%) were positive at the acute onset and 12 out of 14 patients (85.7%) were positive before discharge. In total, 12 out of 14 patients (85.7%) were positive either at acute onset or before discharge. All four atypical KD patients showed positive reactivity. Specific positive bands against recombinant human α‐enolase were detected by western blotting in all four KD patients, but no reactivity was seen in three patients with normal controls. Conclusion: This is the first study to demonstrate that autoantibodies against the α‐enolase are present in the sera of KD patients. We suggest that anti‐α‐enolase Ab should be a good candidate for a diagnostic tool in atypical KD
Full Text
http://informahealthcare.com/doi/abs/10.1080/03009740701607075
DOI
10.1080/03009740701607075
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Soo(김동수)
Lee, Kwang Hoon(이광훈)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/107248
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links